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Development of a Murine Perfusion System

This project focuses on creating a miniature Langendorff perfusion system tailored for murine hearts, enabling controlled drug introduction and effective examination of cardiac responses. The system features temperature control, interchangeable components, and gravity-based flow, emphasizing the importance of heat stability and multi-drug delivery capabilities. The prototype aims for functionality through the use of a Peltier element to maintain perfusate temperature at 37 ± 1°C. Future work will involve the acquisition of additional components, assembly, and testing of the system for cardiac drug delivery applications.

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Development of a Murine Perfusion System

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  1. Development of a Murine Perfusion System Nicola Asgill Fredrick Hilliard George Kittos

  2. Objectives • Langendorff perfusion system • Requirements: • Miniature scale • Fluid temperature controlled • Inter-changeable components • Drug introduction into perfusate

  3. Background • Langendorff Perfusion System • Simulates natural blood flow • Retrograde fluid flow • Attached to aorta • Examine effects of drug on heart

  4. Considerations for project • Why will this work? • Controlled temperature, flow rate and pressure • Improvement of heat stability • Multi-drug delivery capability • Disposable components • Why is this important? • Possible testing applications: • Drug delivery • Cardiac cell response

  5. Prototype

  6. Prototype Modifications for Preliminary Testing • Flow will be gravity controlled • Removal of Peristaltic Pump • Removal of Flow Sensor • Pump and Sensor will be added based on results of preliminary testing

  7. Peltier Element

  8. Peltier Element Test • Goals • Show functionality – Heating capabilities • Calculate Time Constant • To heat from room temperature to 37 ± 1°C • Determine appropriate input voltage

  9. Peltier Element Test • Conclusion: • Input Voltage of 8V – longer time to heat sample, needs to be faster to perfusate at constant temp. • Input Voltage of 15V – faster heating, but found that this voltage will cause the device to fail. • Input Voltage of 12V – faster heating and the device did not malfunction

  10. LabVIEW Temperature Control

  11. Transparent Glass Chamber • Plexiglass • Visualize electric activity in heart using dyes • Staggered holes

  12. Transparent Glass Chamber

  13. Tygon Tubing Peltier Element Current Work • Heating Perfusate to 37 ± 1°C • Manufacturing Plexiglass chamber • Verify and Validate the LabView Module for controlling and maintaining constant temperature

  14. Future Work • Acquiring additional components • Assembling Prototype • Testing the perfusate heating apparatus • Design and Test Control Valve System • Harvesting the Mouse Heart

  15. Thanks Questions?

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