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Development of a Murine Perfusion System

This project aims to create a miniature Langendorff perfusion system tailored for murine models, facilitating precise drug delivery and temperature-controlled fluid flow. The new system will feature interchangeable components and the capability to assess heart function through parameters like blood pressure (BP), electrocardiograms (ECG), heart rate (HR), and cardiac output. Our goal is to enhance the stability of temperature and flow rate, allowing for improved drug testing applications in cardiac research and cell response analysis.

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Development of a Murine Perfusion System

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  1. Development of a Murine Perfusion System Nicola Asgill Fredrick Hilliard George Kittos

  2. Objectives • Langendorff perfusion system • Requirements: • Miniature scale • Fluid temperature controlled • Inter-changeable components • Drug introduction into perfusate

  3. Background • Langendorff Perfusion System • Simulates natural blood flow • Retrograde fluid flow • Attached to aorta • Examine effects of drug on heart

  4. Background (cont.) • Current technology • ADInstruments • Mouse, rat and guinea pig analysis • BP • ECG • HR • Cardiac output • Flow rate

  5. Considerations for project • Why will this work? • Controlled temperature, flow rate and pressure • Improvement of heat stability • Multi-drug delivery capability • Disposable components • Why is this important? • Possible testing applications: • Drug delivery • Cardiac cell response

  6. Prototype

  7. Progress • Established project goals with advisor • Developed progress timeline • Began initial investigations into components/schematics

  8. Future Work • Confirm component selection & system design • Observe perfusion system currently in use • Begin system fabrication

  9. Thanks Questions?

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