Chemistry Shirts 2009: Peptide or periodic table designs also available. Order on Fridays pre-seminar in Chem. Library or from Dr. Raymond, CB 243
Chemistry Seminar F 4/10 3:15 pm SL 130 Do try to attend. This guy is good!
Office Hours this week: M 12-1 R 2-3
Figure 31-1 The induction kinetics of b-galactosidase in E. coli. Page 1217
Total RNA Pulse-chase MVA Fig. 26.3
3 Major Types of RNA: messenger transfer ribosomal Other small RNAs are involved in splicing.
(prok) Inh. initiation Euk inhibitor Blocks elongation MVA Fig. 26.4
Table 31-1 Components of E. coli RNA Polymerase Holoenzyme. Page 1221
RNAP IV!! Table 31-2 RNA Polymerase Subunitsa. Page 1232
Figure 31-11aX-Ray structure of Taq RNAP core enzyme. a subunits are yellow and green, b subunit is cyan, b¢ subunit is pink, w subunit is gray. What do you notice about this structure? Page 1224
Figure 31-13bModel of the open (Rpo) complex of Taq RNAP with promoter-containing DNA showing the transcription bubble and the active site. Page 1225
Figure 31-14 The two possible modes of RNA chain growth. Growth may occur (a) by the addition of nucleotides to the 3¢ end and (b) by the addition of nucleotides to the 5¢ end. Page 1226 How could you distinguish between these two possibilities?
DNA Footprinting http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/F/ Footprinting.html
Figure 31-10 The sense (nontemplate) strand sequences of selected E. coli promoters. Page 1223
Figure 31-12a The sequence of a fork-junction promoter DNA fragment. Numbers are relative to the transcription start site, +1. Page 1225
Figure 31-15 RNA chain elongation by RNA polymerase. Page 1227
Figure 31-16 An electron micrograph of three contiguous ribosomal genes from oocytes of the salamander Pleurodeles waltl undergoing transcription. Page 1228
RNA Backtracking MVA Fig. 26.10
Figure 31-18 A hypothetical strong (efficient) E. coli terminator.