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Blood tubes. Blood tubes. Using the appropriate phlebotomy supplies is imperative for accurate test results. Each vacutainer tube is color-coded to facilitate proper specimen collection. Please be sure to use the proper tube for the desired test. Anticoagulants .
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Blood tubes • Using the appropriate phlebotomy supplies is imperative for accurate test results. • Each vacutainer tube is color-coded to facilitate proper specimen collection. • Please be sure to use the proper tube for the desired test.
Anticoagulants • Hematology testing primarily uses whole blood samples (Blood colection1%BW) • Hemostatic testing uses both whole blood and blood plasma • An anticoagulant is a chemical that , when added to the whole blood sample, prevents or delays clotting (coagulation) of the sample • Regardless of the anticoagulant chosen, the sample and anticoagulant must be well mixed (by gentle inversion0 before use • Samples that are not tested within 1 hour of collection should be refrigerated .
Lavender top Potassium EDTA (K2 or K3 EDTA): For tests requiring EDTA plasma, separate plasma appropriately. Use for CBC and blood cultures Ethylenediamine tetraacetic acid (EDTA)
Green tops • The interior of the tube wall is coated with sodium heparin, lithium heparin, or ammonium heparin • The anticoagulant heparin activates antithrombins, thus blocking the coagulation cascade and producing a whole blood / plasma sample instead of clotted blood plus serum
Green top • Sodium Heparin: Preferred heparin tube for send out testing. • It is glass and Does Not contain inert gel • For plasma determinations in avian and reptiles
Lt. Green top • Lithium Heparin: Contains an inert gel for separating plasma, which acts as a barrier between cells and plasma after centrifugation.
Red top • Contains no anticoagulant. Used in Blood Drug Screens and other testing where the gel additive could interfere with testing. • Red No Gel tubes are available in "No Additive" clot tubes as well as "Clot Activator" tubes for serum collection. • Please take care to inspect the tube label to identify the additive type. • Use for serum determinations in chemistry, serology and Immunohematology (blood banking). • TESTS -SEROLOGY
Red-Gray (tiger) • Contains clot activator and inert gel for separating serum, which acts as a barrier between cells and serum after centrifugation. • During centrifugation the barrier gel moves upward to the serum - clot interface, where it forms a stable barrier separating the serum from fibrin and cells.
Important • For tests requiring serum, a blood sample is drawn into a tube containing no anticoagulant. The blood must be allowed to clot for approximately 30 minutes before centrifugation. • Clotted blood should then be centrifuged for 10-15 minutes. • The serum can then be separated from the cells and transported in an aliquot tube, if necessary. • The use of a transfer pipette is the required method, please DO NOT tilt the original tube when separating the serum into an aliquot tube. • The presence of red blood cells may give erroneous results
It is the responsibility of all personnel who collect laboratory specimens to properly label the specimens to assure accurate patient/sample identification.