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DNA, Chromosomes and the Nucleus

DNA, Chromosomes and the Nucleus

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DNA, Chromosomes and the Nucleus

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  1. DNA, Chromosomes and the Nucleus

  2. DNA Structure

  3. DNA Structure • What are some of the important structural features of the DNA double helix?

  4. DNA Structure • In addition to B-DNA what other forms exist?

  5. DNA Structure • What is supercoiled DNA and what effect does it have? • Can it occur in linear DNA molecules?

  6. DNA Structure • What is the difference between topoisomerases and DNA gyrase? • How can one bring about the denaturation and renaturation of DNA?

  7. DNA Structure

  8. DNA Structure • What is the DNA melting temperature?

  9. Genomic DNA • How do we define the term “genome”? • How is genomic size expressed? • Is there a relationship between genomic size and complexity of the organism or virus?

  10. Genomic DNA

  11. Genomic DNA • What are restriction enzymes and how have they helped us to study DNA?

  12. Genomic DNA • How can restriction fragments be characterized?

  13. Genomic DNA • How is restriction mapping carried out and what information does it provide?

  14. Genomic DNA • How is the chain termination method of Sanger used to determine the sequence of a DNA molecule?

  15. Genomic DNA • What is bioinformatics and proteomics? • How much variation is there in the human genome?

  16. Genomic DNA • How did the work of Britten and Kohne help explain why eukaryotic genomes are so large?

  17. Genomic DNA • What is the difference between tandemly repeated DNA versus interspersed repeated DNA?

  18. Genomic DNA • What are the possible functions of this tandemly repeated DNA? • What about the role of this DNA fingerprinting? • in certain genetic diseases?

  19. DNA Packaging • How is the bacterial genome structured to fit inside of the small cell? • What are bacterial plasmids?

  20. DNA Packaging • What is the relationship between DNA and chromosomes in eukaryotic cells? • What are nucleosomes?

  21. DNA Packaging • What experimental evidence supported the existence of nucleosomes?

  22. DNA Packaging • How is the nucleosome organized? Where is the H1 histone protein?

  23. DNA Packaging • How are nucleosomes packaged to form chromosomes? • What is the DNA packing ratio?

  24. DNA Packaging • How does mitocondrial and chloroplast DNA compare with nuclear DNA?

  25. Nucleus • What do we know about nuclear structure?

  26. Nucleus • What are the major features of the nuclear pore complex?

  27. Nucleus • What kinds of molecules are transported across the nuclear envelope?

  28. Nucleus • How are molecules transported across the nuclear envelope?

  29. Nucleus • What role does the nuclear matrix serve? • What role does the nuclear lamina serve? • What is the function of the nucleolus?