DNA Structure • What are some of the important structural features of the DNA double helix?
DNA Structure • In addition to B-DNA what other forms exist?
DNA Structure • What is supercoiled DNA and what effect does it have? • Can it occur in linear DNA molecules?
DNA Structure • What is the difference between topoisomerases and DNA gyrase? • How can one bring about the denaturation and renaturation of DNA?
DNA Structure • What is the DNA melting temperature?
Genomic DNA • How do we define the term “genome”? • How is genomic size expressed? • Is there a relationship between genomic size and complexity of the organism or virus?
Genomic DNA • What are restriction enzymes and how have they helped us to study DNA?
Genomic DNA • How can restriction fragments be characterized?
Genomic DNA • How is restriction mapping carried out and what information does it provide?
Genomic DNA • How is the chain termination method of Sanger used to determine the sequence of a DNA molecule?
Genomic DNA • What is bioinformatics and proteomics? • How much variation is there in the human genome?
Genomic DNA • How did the work of Britten and Kohne help explain why eukaryotic genomes are so large?
Genomic DNA • What is the difference between tandemly repeated DNA versus interspersed repeated DNA?
Genomic DNA • What are the possible functions of this tandemly repeated DNA? • What about the role of this DNA fingerprinting? • in certain genetic diseases?
DNA Packaging • How is the bacterial genome structured to fit inside of the small cell? • What are bacterial plasmids?
DNA Packaging • What is the relationship between DNA and chromosomes in eukaryotic cells? • What are nucleosomes?
DNA Packaging • What experimental evidence supported the existence of nucleosomes?
DNA Packaging • How is the nucleosome organized? Where is the H1 histone protein?
DNA Packaging • How are nucleosomes packaged to form chromosomes? • What is the DNA packing ratio?
DNA Packaging • How does mitocondrial and chloroplast DNA compare with nuclear DNA?
Nucleus • What do we know about nuclear structure?
Nucleus • What are the major features of the nuclear pore complex?
Nucleus • What kinds of molecules are transported across the nuclear envelope?
Nucleus • How are molecules transported across the nuclear envelope?
Nucleus • What role does the nuclear matrix serve? • What role does the nuclear lamina serve? • What is the function of the nucleolus?