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DNA Barcoding in Germany

History : 2000-2003: DNA TAX (ZSM), marker-hunting … 2006: Cooperation ZSM – Guelph (Lepidoptera) 2009: Barcoding Fauna Bavarica (BFB) 2010: North Sea Project (Senckenberg) 2011: FREDIE Project ( Freshwater , ZFMK; others ) 2012: German Barcode of Life Project (GBOL)

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DNA Barcoding in Germany

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  1. History: 2000-2003: DNA TAX (ZSM), marker-hunting … 2006: Cooperation ZSM – Guelph (Lepidoptera) 2009: Barcoding Fauna Bavarica (BFB) 2010: North Sea Project (Senckenberg) 2011: FREDIE Project (Freshwater, ZFMK; others) 2012: German Barcode of Life Project (GBOL) 2012: GBOL-associates: GeneStream, Lichens, Subterranean Fauna etc. DNA Barcoding in Germany

  2. Lepidoptera Campaign(ZSM) W.G. 1.9 Lepidoptera

  3. Lepidoptera Campaign(ZSM) Period: 2006-2013 Budget: 200K $ assalariesforonescientist ZSM andstudents(State of Bavaria) Associates & experts in Germany: >30 Output: >20,000 species, >65,000 barcodes W.G. 1.9 Lepidoptera

  4. BFB (Barcoding Fauna Bavarica)

  5. BFB (Barcoding Fauna Bavarica) Period: 2009-2013 Budget: 1 Mio $ + Salaries (Bavaria) Principleinvestigators: 6 scientists ZSM Associates & experts: 50 (MEG) Target: All Animals of Bavaria (35,000) Output: 12,000 species, 55,000 barcodes

  6. BFB (Barcoding Fauna Bavarica) others 3000 Arach. Myriap. 1400 Coleoptera 15,000 Heteroptera 1600 Diptera 3000 Hymenoptera 16,000 Lepidoptera 15,000 55,000 barcodes

  7. BFB (Barcoding Fauna Bavarica) others 1150 Coleoptera 3,600 Arach. Myriap. 600 Heteroptera 500 Diptera 1000 Hymenoptera 2,400 Lepidoptera 2,950 >12,000 species

  8. GBOL (German Barcode of Life project)

  9. GBOL (German Barcode of Life project) Period: 2012-2015 Budget: 7.1 Mio $ (German Ministryfor Science) + Salaries Principleinvestigators: 17 (mainly ZFMK, ZSM, other 7 partners) Associates & experts: 171 Target: All animals & plantsof D (50,000) Output: 5.300species, 17,000barcodes

  10. GBOL (German Barcode of Life project) 1,200 Coleoptera 1,000 Lepidoptera 800 Hymenoptera 17K barcodes 5.3K species

  11. North Sea Project Period: 2010-2016 Budget: 2.9 Mio $,onlypartimfor DNA Barcoding (German MinistryforScience) Principleinvestigators: 6 (DZMB Senckenberg) Associates & experts: 5 Target: Marine Animals of North Sea Output: 400 species, 3,000 barcodes (focusCrustacea)

  12. FREDIE Period: 2011-2014 Principleinvestigators: 18 (ZFMK, IGB, MNHU) Associates & experts: 55 Target: European freshwateranimals Output: 10,000 barcodes, 1,300 species (focus onfishes, mayflies)

  13. GBOL associates(Lichens, ‚genestream‘, subterraneanfauna) Period: 2012-2015 Budget: directedfrom GBOL forpilotstudies (approx. 50K $) with additional matchingfundsfromuniversities Principleinvestigators: 3 (BSMunich, Uni Frankfurt, Uni Bochum), about 30 associateexperts Target: Lichens, freshwaterinsectsandsubterraneananimalsof Germany Output: 20 Lichen species (phyto- vsmykobionts), 20 troglodyphilousspeciesand 50 freshwaterinsectspecies

  14. ABA (AcceleratedBiodiversity Assessment) Period: 2010-2013 Budget: 2+ Mio$ (?), onlypartimfor DNA Barcoding (DFG) Principleinvestigators: 3 botanists, 1 coleopterologist Target: Fungi, plants, Coleoptera in Ecuador Output: ??

  15. SpongeBarcoding Period: 2010-2011 Budget: 200K $ (DFG/Sloan Foundation) Principleinvestigators: 3 (LMU Munich, Wörheide) Target: spongesworldwide Output: 1000 COI sequences (+ othermarkers), 100+ species

  16. DNA Bank facilities Period: 2010-2012 Budget: approx. 2.8 Mio $ (DFG) Principleinvestigators: >10 (ZSM, ZFMK, BGBM, DSMZ) Target: DNA storage Output: storageof >100K aliquots

  17. Summary • Total investmentof Germany • directedtoiBOL DNA Barcode Library: • 11.4 Mio $ • additional associateprojectfunds(not directlyto DNA Barcode Library) • 7.8 Mio $

  18. PastBarriers • Freshmuseum material for150+K insectspeciesfromtropical countries in German museums • Barcode pipelineestablished • Lead ofmuseumsveryiBOL-friendly, barcodingbecame a majorfocus in severalinstitutions BUT: problemstoraisefundsfortissuecollecting, databasingandsequencingof non-European material

  19. PastBarriers • insufficient BOLD-taxonomyformanytropicalinsects • missing large-scaleprogramsforefficientcalibration Barcoded holotypes from the collection Herbulot (ZSM)

  20. Fundingopportunities • BFB2 (starting 2014) another 1 Mio $ in discussion • GBOL2 (starting 2015) another 7 Mio $ planned • German/IndonesianprogramBiodiversity & Health(2014-2020); ‚anti-infectivesubstances‘): 2-3 Mio $ forspeciesdescriptionpipelinebased on DNA barcodes (100K barcodes / 30K species) • ATSB (starting 2014) 200K $ forassessmentof non-targets in African Malaria project • contribute material to international programs

  21. Explorative barcoding of megadiverse insect taxa: The hidden diversity of microhymenoptera Chalcid wasps (Hymenoptera: Chalcidoidea)

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