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Protein Quantitation Methods in HPLC Analysis

Explore protein quantitation using various modes of HPLC and calibration curves for accurate measurements. Learn about ideal and non-ideal SEC modes, day-to-day precision, antibody analysis, and column comparisons.

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Protein Quantitation Methods in HPLC Analysis

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  1. Figure S1 – Calibrationcurve SEC ideal mode Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 1

  2. Figure S2 – SEC non ideal mode, day-to-dayprecisionareaofmyoglobinandovalbuminon BioSep™column Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 2

  3. Figure S3 –SEC ofIgGantibody; retentiontimes Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 3

  4. Figure S4 –SEC ofIgGantibody; areaof dimer Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 4

  5. Figure S5 –SEC ofIgGantibody; areaof monomer Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 5

  6. Figure S6 –RP controlchartofpeakmyoglobin 2, day 3 Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 6

  7. Figure S7 – Separation ofmyoglobin, ovalbuminand BSA in SAX modeat different wavelength Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 7

  8. Figure S8 –RP blank injection, chemicalnoise Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 8

  9. Figure S9 –SAX blank injection Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 9

  10. Figure S10 – WCX blank injection Protein Quantitation using various modes of HPLC l Grotefend, Kaminski, Wroblewitz, El Deeb, Kühn, Reichl, Limberger, Watt, Wätzig l Page 10

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