Silver Staining Protocol for Protein Separation and Visualization in Electrophoresis

Dec 13, 2025

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This document outlines a detailed protocol for silver staining of proteins separated by electrophoresis. The method employs a 12% SDS-PAGE system and an IEF (Isoelectric Focusing) setup, tailored for effective visualization of low-abundance proteins. The poster provides a clear step-by-step guide for preparing reagents, applying the silver stain, and interpreting the gel results. This technique enhances protein detection sensitivity, making it an essential tool in biochemistry and molecular biology research.

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Silver Staining Protocol for Protein Separation and Visualization in Electrophoresis

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