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Analysis of PHF Phosphorylation and Ubiquitination Sites in Isoform 2: A Comprehensive Overview

This study presents a detailed analysis of phosphorylation and ubiquitination sites in the PHF protein isoform 2. Using advanced techniques such as LysC digestion, we identified various phosphorylation residues and explored their sequence coverage. Notably, we discovered no ubiquitination sites using LysC digestion due to its cutting pattern. Our findings contribute to a better understanding of PHF post-translational modifications, their implication in cellular processes, and their potential as therapeutic targets.

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Analysis of PHF Phosphorylation and Ubiquitination Sites in Isoform 2: A Comprehensive Overview

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  1. Tau Dataset Analysis

  2. PHF tau phosphorylation and ubiquitination sites (isoform 2) P MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQT PTEDGSEEPG SETSDAKSTPTAEDVTAPLVDEGAPGKQAA AQPHTEIPEG TTAEEAGIGDTPSLEDEAAGHVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPK SGDRSGYSSP GSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPM PDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSK CGSKDNIKHV PGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMVDSPQLATLADEVSASLAKQG L P P U U P P P P P P P P P P U P P U P U U U U P U P P U U P P P P P BLUE = sequence coverage; = phosphorylation; = ubiquitination P U

  3. Ubiquitin Sequence 6 11 MQIFVKTLTG KTITLEVEPS DTIENVKAKI 18 QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN 63 IQKESTLHLV LRLRGG

  4. K6: MQIFK@TLTGK K11: TLTGK@TITLEVEPSDTIENVK K48: LIFAGK@QLEDGR K63: TLSDYNIQK@ESTLHLVLR For LysC digestion, we didn’t identify any ubiquitation sites since LysC doesn’t cut on R.

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