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Bioinformatics for Vet. Part XII

Bioinformatics for Vet. Part XII. Sung Youn Lee, PhD. Student Veterinary collage, Room 320 02 450 3719, 016 293 6059 leevet@paran.com. GC-content In genetics, GC-content ( guanine-cytosine content ) is a characteristic of the genome

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Bioinformatics for Vet. Part XII

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  1. Bioinformatics for Vet. Part XII Sung Youn Lee, PhD. Student Veterinary collage, Room 320 02 450 3719, 016 293 6059 leevet@paran.com

  2. GC-content In genetics, GC-content (guanine-cytosine content) is a characteristic of the genome of any given organism or any other piece of DNA. It is the proportion of GC-base pairs in the DNA. G stands for guanine and C stands for cytosine. GC-pairs in the DNA are connected with three hydrogen bonds instead of two in the AT-pairs (adenine and thymine). This makes the GC-pair stronger and more resistant to denaturation by high temperatures. The GC-content is sometimes used to classify organisms in taxonomy, for example, the Actinomycetales bacteria are characterised as "high GC-content bacteria". In Streptomyces coelicolor it is 72%. The GC-content of Yeast (Saccharomyces cerevisiae), for example, is 38%, that of another common model organism Thale Cress (Arabidopsis thaliana) is 36%. In PCR the GC-content of primers is used to determine annealing temperature.

  3. Effect of GC content on Tm

  4. Working with a Single DNA Sequence • Proteins perform most biological functions, so biologists tend to consider DNA sequence kind of dull. • Please remind about ‘Central Dogma’. • DNA sequences are the mothers of all sequences.

  5. Analyzing DNA composition • Establishing the G+C content of ur sequence • http://www.artsci.wustl.edu/~twest/molbio/gccontent.php

  6. Thank you for your attention ~

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