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Introduction to Vector NTI: A Comprehensive Guide for Molecular Biology

This guide provides an overview of Vector NTI Advance 10.0, an essential software suite for molecular biology professionals. It covers how to import or create new sequences, perform sequence alignments, and conduct sequence assembly with ContigExpress. The guide also includes practical exercises such as cloning a fragment from pBR322 to pUC19 and designing PCR primers for the Epidermal Growth Factor Receptor (EGFR), a significant biomarker in cancer research. For further resources, it links to informative PowerPoint guides and tutorials.

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Introduction to Vector NTI: A Comprehensive Guide for Molecular Biology

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  1. Introduction to VectorNTIOctober 1, 2008 Ansuman Chattopadhyay, PhD, Head Molecular Biology Information Service Health Sciences Library System University of Pittsburgh ansuman@pitt.edu http://www.hsls.pitt.edu/guides/genetics

  2. Topics • Vector NTI Advance 10.0 Suite Overview • How to Import or Create New Sequences • Sequence Alignments: AlignX • Sequence Assembly: ContigExpress • Analyze Sequences: PCR and Primers, Restrictions and Mutagenesis • How to create Vector Map

  3. Exercise • PLCg1: • Mouse: NP_067255 • Rat: NP_037319 • Cow: NP_776850 • Dog: XP_542998 • Zebra fish: NP_919388 • Human: NP_002651

  4. Important Links • PowerPoint Guide to Molecule Construction and Design • PowerPoint Guide to Managing the Vector NTI Database • Northwestern University VectorNTI tutorial

  5. A Molecule Construction Example • Task – Clone a fragment from pBR322 into pUC19 ☼Donor fragment: pBR322, 5’EcoRI—3’AvaI ☼Recipient fragment: pUC19, 5’SmaI—3’EcoRI • Getting started 1. Open pBR322 and pUC19 in Vector NTI 2. Arrange the display window to display 2 molecules on the same screen: ☺Select Menu > Window > Tile Vertical

  6. Hands - On • Epidermal Growth Factor receptor is considered as one of the bio-markers for several forms of cancer including Breast and Ovarian cancers. • Create a Vector NTI file for the gene EGFR (Refseq Accession Number: P00533 ) • Design PCR primers to amplify any one domain of your choice present in EGFR protein.

  7. Thank you!Any questions? Carrie Iwema Ansuman Chattopadhyay iwema@pitt.eduansuman@pitt.edu 412-383-6887 412-648-1297 http://www.hsls.pitt.edu/guides/genetics

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