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Development of Local and Scanning Probe Techniques

Development of Local and Scanning Probe Techniques

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Development of Local and Scanning Probe Techniques

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  1. Development of Local and Scanning Probe Techniques Heinrich Hoerber NanoBioPhysics University Bristol

  2. Combining AFM and optical microscopy Haberle, W., J. K. H. Horber, and G. Binnig, 1991, Journal of Vacuum Science & Technology B 9, 1210 200 nm

  3. AFM image sequence of a pox virus release at the end of a microvillus Scanning fast - making movies EM image by Stokes 1976 Horber, J.K.H., et al., 1992, Scanning Microscopy 6, 919

  4. Combination with electro-physiological techniques Horber, J. K. H., W. Haberle and B. Sakmann, 1995 Biophysical Journal 68, 1687

  5. AFM in hearing research Koitschev, A., S. Fink, U. Rexhausen, K. Loffler, J. K. H. Horber, H. P. Zenner, J. R. Ruppersberg, and M. G. Langer, 2002, Hno 50, 464-469

  6. Cantilever development IBM Research Laboratory Rueschlikon M. Despont, G. Binnig, P. Vettiger and C. Gerber

  7. Heat transfer through cantilever arms (~4 mW) Heat flow between cantilever and substrate 50-5000C Cantilever with heater through tip in contact 50-500 nW ΔR/R: 10-6–10-5/nm Through air to substrate (10-20 μW)

  8. SiC GaN Heat conductivity 2 μm 1 μm 2 μm Lipid vesicles with reconstituted membrane proteins (SNAP25, B.Jena) Metal connection on a storage chip structure underneath a SiO2 layer (Zarlink) Cut through a transistor structure (M. Kubal) Haeberle, W, Pantea, M & Hoerber, JKH. 2006, Ultramicroscopy, 106 (8-9), 678

  9. length 260 m, width 1 m, thickness 200 nm, spring constant 0.03 pN/nm Smaller cantilevers 100 m 1 m James Vicary

  10. AFM with the cantilever vertical Massimo Antognozzi, ArturasUlcinas

  11. Maximal cantilever bending Molecular Motor movement Massimo Antognozzi, Tim Scholz

  12. 200 μm 0.5 nm 0.02 N/m 200 nm Photonic Force Microscopy Spatial resolution ~ 1nm Time resolution ~ 1μsec Florin, E. L., A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, 1998, Applied Physics A-Materials Science & Processing 66, S75

  13. Agarose polymer network Tischer, C. et al., 2001, Appl. Phys. Lett. 79, 3878 Thermal fluctuation imaging

  14. Membrane diffusion 2D / 3D

  15. Interaction map of an LDL receptor Viscosity map Confining potential map

  16. Parallelisation of PFM

  17. Producing many independent focused laser beams

  18. Nano-particles crossing the cell membrane

  19. Types of nano-particles

  20. Gold nano-particles interacting with light

  21. Nearfield fluorescence excitation

  22. Surface Enhanced Raman Spectroscopy Chemical sensing Raman Spectroscopy

  23. Nano-particles - areas of interest • Nano-Sensors (Scanning Probe Microscopies) • Nano-toxicology • Nano-medicine (markers, drug delivery)