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Optimizing Split and Splitless Injections in Analytical Methods

This guide explores the critical aspects of split and splitless injections in chromatography, highlighting various flow rates like 2 ml/min, 5 ml/min, and 100 ml/min. We'll discuss split ratios, including 25:1 and 24:2, and detail how these parameters affect retention factors and compound interaction with stationary phases. Additionally, it provides insights into temperature programming and peak overload issues, enhancing your analytical techniques for improved efficiency and accuracy. For more tips, visit our resource page.

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Optimizing Split and Splitless Injections in Analytical Methods

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  1. GC setup

  2. 2 ml/min 2 ml/min 2 ml/min 103 ml/min 28 ml/min 28 ml/min 24 ml/min 25 ml/min 100 ml/min Split ratio 25:1 Split ratio 24:2 Split ratio 100:1 2 ml/min 1 ml/min 1 ml/min SPLIT Injections www.kinesis-solutions.co.uk/kinesis_techtips.asp?id=11

  3. SPLIT Injections

  4. Peak overload www.chem.agilent.com/cag/cabu/capgccols.htm

  5. 2 mL/min 2 mL/min 3 mL/min 5 mL/min 1 mL/min 3 mL/min SPLITLESS Injections X www.kinesis-solutions.co.uk/kinesis_techtips.asp?id=11

  6. SPLITLESS Injections

  7. DIRECT Injections

  8. Stationary phase interaction

  9. Types of Stationary phases

  10. Temperature Program

  11. Temperature Program

  12. Retention Factor Describes the strength of the interaction of a compound with the stationary phase tR-tM VR-VM k’ = = tM VM 1 < k’ < 10

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