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Extracting Biologically active substances from Plants

This research project conducted by students Haruna Kobayashi, Yohei Nosaka, Yuta Fukushima, and Eriko Minamiya at Koshi High School explores the extraction of biologically active substances from plants, inspired by their seniors' study on chemical-free fertilizers. Using methods like thin layer chromatography (TLC) and column chromatography, the team investigated components from various plants, including Cinnamon, Rooibos, and Chaga fungus. Preliminary results show promising concentrations of bioactive compounds that may have practical applications in health and wellness.

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Extracting Biologically active substances from Plants

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  1. Extracting Biologically active substancesfromPlants KoshiHigh School Haruna Kobayashi YoheiNosaka Yuta Fukushima Eriko Minamiya

  2. ~Motive~ We listened to our seniors’ study “Making chemical-free fertilizer with plant components” and we will extract component from plants to find good applications for our life.

  3. ~Research Material~ Cinnamon Cork Tree Bracket fungus Chaga Rooibos

  4. Cinnamon Cork tree ・Spice ・We say it has hyperthermicaction. ・Rutaceae Rooibos ・we say it is a medicine of perpetual youth and long life ・It has antioxidant action

  5. Chaga Bracket fugus ・Shelf-type mushrooms ・It is born in the stem of natural trees, fallen trees, and dead trees ・Cold-resistant mushroom ・It is born in cold areas

  6. Bracket fugus and Chaga are relatives. But both of them are different in trees. Do they have different components!?

  7. ～First Experiment ～ Crushmaterial with ethanol using a machine. Leave it forthree weeks. Concentrate it. Add water to it, to get n-Hexane Extracts.

  8. ～Thin Layer Chromatography(TLC)～ ～Method～ ①Make 10ml of solution which is 90% Chloroform and 10% Methanol (we will just call it “the solution” after this), and make a sample with the solution. Vacuum Filtration Concentration

  9. ②Spotthebottom of the TLC plate with the concentrate from step ①. Then, we put 5ml of the solution into a glass case and we put the TLC into the case.

  10. ③After the solution rises enough, check if you can see component spots withUVlight.

  11. ～Results of Experiment～ The line of rising solution Ⅲ Bracket fugus ⅠCinnamon Ⅱ Rooibos ⅣCork tree, Chaga …The first spot …Spots of components （A thicker mark means the component is more concentrated.）

  12. ～Column Chromatography～ ～Method～ ① Make 200ml of the solution ② Put Silica gel and the solution into an Erlenmeyer flask and mix them. Chloroform Methanol Silicagel

  13. ③ Put ② into a column and pour in a little solution. • After that we put 5ml of the solution mixed with a sample into the Column .

  14. ④ Put a little Silica gel on ③and pour little by little another 200ml of the solution. Mixed Silica gel and 5ml solution → Expand → Silica gel →Sample →Solution

  15. ⑤ Divide the liquid into 2 equal parts, put in a Flask and concentrate them.

  16. Result of Experiment • Ⅰ:Cinnamon

  17. Ⅱ:Rooibos Ｒ－０ Ｒ－１ Ｒ－２

  18. Ⅲ：Bracket fungus Ｓ－１ Ｓ－２ Ｓ－３ Ｓ－４ Ｓ－５ ※There is not S-0 picture.

  19. C－１ C－２ C－３ R－１ R－２ S－１ S－０, ２～５ R－０ C－０ Ⅰ Ⅱ Ⅲ • ……The first spot • …Spot of components （a thicker mark means the spot is more concentrated）

  20. ～Consideration～ Cinnamon ・C-2 has a strong C-0 smell ⇒We think C-2 has fragrance components. ・C-3 has C-0 color ⇒We think C-3 has color components. • Rooibos ・R-2 is like C-0 color ⇒We think R-2 has color components. • Bracket fugus • ・S-2 is like S-0 smell • ⇒We think S-2 has fragrance components.

  21. ～Future Activities～ ・Try to do other experiments . ・Try to extract component of other plants. ・Conduct experiments to test the effects of components. We will examine whether Rooibos have inhibits UV light and so on.

  22. Thank you f r listening o

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