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Extracting Biologically active substances from Plants

Extracting Biologically active substances from Plants . Koshi H igh School. Haruna Kobayashi Yohei Nosaka Yuta Fukushima Eriko Minamiya. ~Motive~.

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Extracting Biologically active substances from Plants

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  1. Extracting Biologically active substancesfromPlants KoshiHigh School Haruna Kobayashi YoheiNosaka Yuta Fukushima Eriko Minamiya

  2. ~Motive~ We listened to our seniors’ study “Making chemical-free fertilizer with plant components” and we will extract component from plants to find good applications for our life.

  3. ~Research Material~ Cinnamon Cork Tree Bracket fungus Chaga Rooibos

  4. Cinnamon Cork tree ・Spice ・We say it has hyperthermicaction. ・Rutaceae Rooibos ・we say it is a medicine of perpetual youth and long life ・It has antioxidant action

  5. Chaga Bracket fugus ・Shelf-type mushrooms ・It is born in the stem of natural trees, fallen trees, and dead trees ・Cold-resistant mushroom ・It is born in cold areas

  6. Bracket fugus and Chaga are relatives. But both of them are different in trees. Do they have different components!?

  7. ～First Experiment ～ Crushmaterial with ethanol using a machine. Leave it forthree weeks. Concentrate it. Add water to it, to get n-Hexane Extracts.

  8. ～Thin Layer Chromatography(TLC)～ ～Method～ ①Make 10ml of solution which is 90% Chloroform and 10% Methanol (we will just call it “the solution” after this), and make a sample with the solution. Vacuum Filtration Concentration

  9. ②Spotthebottom of the TLC plate with the concentrate from step ①. Then, we put 5ml of the solution into a glass case and we put the TLC into the case.

  10. ③After the solution rises enough, check if you can see component spots withUVlight.

  11. ～Results of Experiment～ The line of rising solution Ⅲ Bracket fugus ⅠCinnamon Ⅱ Rooibos ⅣCork tree, Chaga …The first spot …Spots of components （A thicker mark means the component is more concentrated.）

  12. ～Column Chromatography～ ～Method～ ① Make 200ml of the solution ② Put Silica gel and the solution into an Erlenmeyer flask and mix them. Chloroform Methanol Silicagel

  13. ③ Put ② into a column and pour in a little solution. • After that we put 5ml of the solution mixed with a sample into the Column .

  14. ④ Put a little Silica gel on ③and pour little by little another 200ml of the solution. Mixed Silica gel and 5ml solution → Expand → Silica gel →Sample →Solution

  15. ⑤ Divide the liquid into 2 equal parts, put in a Flask and concentrate them.

  16. Result of Experiment • Ⅰ:Cinnamon

  17. Ⅱ:Rooibos Ｒ－０ Ｒ－１ Ｒ－２

  18. Ⅲ：Bracket fungus Ｓ－１ Ｓ－２ Ｓ－３ Ｓ－４ Ｓ－５ ※There is not S-0 picture.

  19. C－１ C－２ C－３ R－１ R－２ S－１ S－０, ２～５ R－０ C－０ Ⅰ Ⅱ Ⅲ • ……The first spot • …Spot of components （a thicker mark means the spot is more concentrated）

  20. ～Consideration～ Cinnamon ・C-2 has a strong C-0 smell ⇒We think C-2 has fragrance components. ・C-3 has C-0 color ⇒We think C-3 has color components. • Rooibos ・R-2 is like C-0 color ⇒We think R-2 has color components. • Bracket fugus • ・S-2 is like S-0 smell • ⇒We think S-2 has fragrance components.

  21. ～Future Activities～ ・Try to do other experiments . ・Try to extract component of other plants. ・Conduct experiments to test the effects of components. We will examine whether Rooibos have inhibits UV light and so on.

  22. Thank you f r listening o

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