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This overview details advanced techniques in motif detection and screening of intergenic sequences using tools like Sequence Blast and Gibbs Motif Detection. The methodologies outlined include phylogenetic footprinting and motif scanning through genome-wide screening approaches. The insights provided aim to assist researchers in identifying homologs inclusively and understanding the regulatory frameworks governing gene expressions, particularly in relation to specific motifs. Additional focus is on regulatory genes such as cydA and yejG as examples of motif relevance.
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Motif detection and Screening 09112006
OVERVIEW Sequence Blast Identify homologs INCLUsive Select Intergenics Motif Sampler/ RSA Phylogenetic footprinting Motif Scanner/ RSA Genome wide screening
OVERVIEW Sequence Blast Identify homologs INCLUsive Select Intergenics Motif Sampler/ RSA Phylogenetic footprinting Motif Scanner/ RSA Genome wide screening
Motif Detection: Gibbs Consensus: TTGATCTAGATCAA Regular expression
OVERVIEW Sequence Blast Identify homologs INCLUsive Select Intergenics Motif Sampler/ RSA Phylogenetic footprinting Motif Scanner/ RSA Genome wide screening
MotifScanner score consensus hit start stop
EcoCYC cydA encodes a terminal oxidase Its regulation by FNR was to be expected
EcoCYC yejG is a novel protein with unknown function But from in silico analysis you know it might be regulated by O2