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This document outlines key embryological stages following fertilization in the female reproductive system. It begins with the release of the egg, its journey through the fallopian tube, and the fusion of sperm and egg nuclei resulting in a zygote. The text details subsequent cleavage stages—2-cell, 4-cell, and 8-cell stages—highlighting differences between protostomes and deuterostomes. Further stages include the morula formation and development of the blastula, followed by the gastrula stage where germ layers are established: ectoderm, mesoderm, and endoderm, crucial for organ formation.
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Embryological Stages March 23, 2011
Fertilization: 0 hours • Egg is released from the ovary & moves into the fallopian tube to wait for the arrival of the sperm • Fertilization- results in a zygote • requires the sperm & egg nucleus to fuse • Following fusion, egg plasma membrane undergoes changes that prevent entrance of other sperm • Then wave-like action of cilia move the fertilized egg toward the uterus for implantation to take place
Cleavage 2-cell: 24 hours • Cleavage: process by which a zygote divides by mitosis to form two new cells • mitosis and DNA replication occur repeatedly • Cell is no longer just one cell
4-Cell: 36 hours • Cleavage continues as two cells will divide to form 4 cells, and so on • Continue dividing until form a blastocyst
8-Cell: 48 hours 8-CELL STAGE IS KEY DIFFERENCE!!! • Either Protostomes or Deuterostomes depending on species • Protostomes (clams, worms, & insects) undergo spiral cleavage- cells divide and they do not sit on top of each other; they appear to spiral
Deuterostomes • Deuterostomes- (starfish & vertebrates) cleavage results in 8 cells sitting directly on top of each other. This is called radial cleavage
Morula 16-Cell: 60-84 hours • The eight cells now continue to divide until you are simply a large solid ball of cells (16-32 cells). Morula
Blastula: 6 days • At this time, the morula begins to form a hollow ball of cells called the blastula. • The blastula contains a fluid filled space known as the blastocoel. BLASTOCOEL BLASTULA
Gastrula: ~15 days • Blastula begins to fold inward on one side • When the folding occurs, it creates a horse shoe shaped structure that is 2 cell layers thick • Outer layer of the ball is called the ectoderm. • Inner layer of the ball is called the endoderm. • Eventually, another cell layer will begin to form between the endoderm and ectoderm. This layer is called the mesoderm. Mesoderm
3 Tissue Layers In Depth • Ectoderm- forms nervous system & outer layer of skin • Mesoderm- forms muscle, bone, & cardiovascular system • Endoderm- organs of digestive system, lining of respiratory system & bladder
Gastrula (cont.) • The point where the horse shoe almost touches is called the blastopore (small hole) • In protostomes this will eventually form the mouth. • In deuterostomes this will form the anus Mesoderm