Exploring Enzyme Kinetics: Understanding Reaction Rates and Mechanisms

This lab session focuses on the principles of enzyme kinetics, emphasizing how enzymes facilitate biochemical reactions. Through a series of experiments, students will measure enzyme activity, analyze factors influencing reaction rates, and determine parameters such as Km and Vmax. By the end of the lab, participants should be able to understand the Michaelis-Menten model, differentiate between competitive and non-competitive inhibition, and apply their knowledge to real-world biological processes.