1 / 9

DNA Profiling Using PCR

DNA Profiling Using PCR. Sara Small, Sarah Petroni , Annelise Yackow. Short Tandem Repeats (STRs). Also known as microsatellites. Short, repeating pieces of 2-6 base pairs. They are non-coding and scattered throughout the genome.

leroy-wright
Télécharger la présentation

DNA Profiling Using PCR

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. DNA Profiling Using PCR Sara Small, Sarah Petroni, AnneliseYackow

  2. Short Tandem Repeats (STRs) • Also known as microsatellites. • Short, repeating pieces of 2-6 base pairs. • They are non-coding and scattered throughout the genome. • Each individual has a varying number of repeated STRs. Each STR can be repeated up to 100 times.

  3. STR/Microsatellite

  4. Process of DNA Profiling 1. Obtain a DNA sample: • Collect tissue from a living or dead organism. • This tissue is then treated with chemicals and enzymes to extract the DNA from the sample. • Only a minimal amount of DNA is necessary.

  5. Process of DNA Profiling 2. Amplify the STR regions using PCR: • Specific primers attach to each end of the STRs. These primers make large quantities of each region. • No other part of the DNA is amplified or replicated. • This process only takes around five minutes.

  6. Process of DNA Profiling 3. Gel Electrophoresis: This process separates the fragments by length. The smaller fragments travel faster than the longer fragments. At this point in the process, it is easy to calculate the length of each STR region and the number of repeat STRs at each site. From here, one can analyze the results of DNA electrophoresis. Lanes with many regular bands are used for calibration, determining the length of fragments in unknown samples.

  7. Use in Criminal Investigations • In criminal investigations, thirteen STR regions are analyzed. To be convicted, a criminal’s DNA must match the crime scene DNA at all thirteen regions. • The odds of another person sharing the same result are about one in a billion.

  8. Other Uses of DNA Profiling • Genetic relatedness such as paternity testing, checking animal pedigrees, or searching for a specific gene in disease screening.

More Related