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Cardiac Morphology, Gene Expression and Histology (Core D)

Cardiac Morphology, Gene Expression and Histology (Core D). Description of Core Unit: Generate staged mouse and Xenopus embryo RNA samples. Generate paraffin and OCT embedded tissues and embryos. Share expertise in and perform some whole mount and section in situ hybridizations.

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Cardiac Morphology, Gene Expression and Histology (Core D)

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  1. Cardiac Morphology, Gene Expression and Histology (Core D) • Description of Core Unit: • Generate staged mouse and Xenopus embryo RNA samples. • Generate paraffin and OCT embedded tissues and embryos. • Share expertise in and perform some whole mount and section in situ hybridizations. • Perform IHC and histology on tissue and embryo sections.

  2. Core D Progress Report I • Successful use of RT-PCR to verify embryonic expression of Xenopus Mo2L mRNA (Tiziana De Angelis). • Next: in situ hybridizations (ISH; mouse and Xenopus; Projects 4 & 5, Ning Dai and Tiziana De Angelis) including Mo2L and mouse Ehmt1 (Project 4). • Control probes for mouse in situs obtained from Tom Lufkin. • Successful sub-cloning of Xenopus LKB1/XEEK1 in KmRN3P.

  3. Core D Progress Report II Additional histological materials generated to date (including NIH Progress Report): • 1518 slides stained. • 1947 slides unprocessed. • Total no. of slides: 3465. • 245 embedded tissue blocks. • 239 processed blocks. • Total no. of blocks: 484. Ning Dai

  4. Interactions with other projects are welcome.

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