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Medaka 09

Medaka 09. Kelsey Burkett and Becca Miles. Before we begin, useful information – parts of Medaka. filament – the filament is a threadlike appendage chorion - a membrane that protects around the egg

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Medaka 09

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  1. Medaka 09 Kelsey Burkett and Becca Miles

  2. Before we begin, useful information – parts of Medaka • filament – the filament is a threadlike appendage • chorion- a membrane that protects around the egg • cytoplasm - this is the gel like gaps where cell respiration and expansion take place around the in the spaces of the Medaka. • yolk sac - moves the nutrients from the yolk to the embryo in developing stages. • oil globule - supplies the egg with nutrients, they look like air bubbles almost • heart - Circulates the blood through the Medaka egg • pectoral fin - fin on the stomach of the Medaka • caudal - fin attached to the tail of the Medaka • dorsal - fin attached to the back of the Medaka • optic cup - develops into the pigmented and sensory layers of the retina • eye - allows the organism to see and sense its surroundings • brain - controls the central nervous system, processes everything throughout the body, the control system. • mouth/jaws - consumes nutrients and begins digestion process by crushing or belittling food before entering the organism • somites- chunks of the mesoderm, found along the neural tube of the embryo, look like little hairs • vitellinemembrane - it is a see through membrane that surrounds the vitellus of the embryo • blood vessel - moves blood throughout the organism • urinary bladder - organ that stores the urine before it leaves • swim bladder - stores the animals gases... • spleen - stores some blood, also can renew/clean the blood • liver - organ in the digestive system, stores glycogen, decomposition of red blood cells, plasma protein synthesis, hormone production, and detoxification.intestine- digests the food an organism eats, pulling out the nutrients needed, before leaving the body

  3. Medaka Survival Requirements • It can move between salt and freshwater • It is found in both ocean and river habitats. • It can withstand cold easily • Usually lives up to at least 4 years or more • The best temperature range for Medaka is between 21 and 26 degrees Celsius • Commonly feed on mosquito larvae normally

  4. In a pond far far away….

  5. Caleb on Day 1 • STAGE: 22 • HOURS: about 40

  6. Day 1 Age reason • stage 22 cause you can see a little bit of the heart, begin to see the yolk sac, • don't think it's to much further only cause the lenses are not fully growing • The eyes are not clearly defined yet • Therefore it can’t be on stage 23. • Shares the same characteristics stage 22 requires and fits the appropriate time line.

  7. Day 2 • STAGE: 26AGE: 2 days and 6 hrs.

  8. Day 2 Age reason • Observed a reddish liquid flowing through the Medaka • In stage 26 blood is said to be pumped out beyond the anterior region of the hind-brain • The tip of the tail is supposed to be completely free of the yolk sphere (we believe it is) • The liver is still in development mode and isn't clearly visible yet. • The eyes begin to darken

  9. Day 3 • STAGE: 29AGE: 3 days, 2 hours

  10. Day 3 Age reason • In stage 29, the embryonic body is supposed to encircle about 3/4 of the yolk sphere. • You should also begin to see the pectoral fin and the membranous fins should become slightly seen in the tail. • The anterior tip of the notochord is located where the branches of the dorsal aorta join.

  11. Day 4 • STAGE: 31AGE: 3 days, 23 hours

  12. Day 4 Age reason • The large cells of the hatching enzyme gland are migrating up to the region under the central part of the eye. • The eye has now become the cornea, blood circulates in the body and is also seen in the gill arches • Pigmentation of the melanophores in the choroidea proceeds as a dark network in the eyes. • The kidney should begin to appear • The gallbladder should also become some what seen even though it is transparent and colorless, it's around the liver too. • The membranous fin on the tail is wider and grew some since the last day.

  13. Day 5 • STAGE: 33AGE: About 4 days, and 10 hours

  14. Day 5 Age reason • The tail tip has not yet reached to the point of the eye. • The notochord is completely vacuolated to the end of the tail. • The tips of the membranous margins of the pectoral fins reach the 4th somite as well. • The pictures on the site http://www.embl-heidelberg.de/mepd/medakaStages/MedakaDevStages.html resemble the same formation as our Medaka egg does at this stage. • I believe since they have the same form and growth rate, that stage 33 should be the correct stage I think.

  15. Day 6 • STAGE: 35AGE: 5 days and 12 hours

  16. Day 6 Age reason • The tip of the caudal fin reaches beyond the posterior border of the eye. • Guanophoresare distributed from the head to the vicinity of the tail tip. • Blood should begin to circulate through the internal tissues of the head and the viscera to Cuvier's ducts of the Medaka. • You should also be able to begin to see the opening of the mouth.

  17. Day 7 • STAGE: 36AGE: 6 days

  18. Day 7 Age reason • This is the stage where the heart should begin to develop fully. • The tip of the tail reaches the optic vesicle. • The extent of flexion of the atrio-ventricular region of the heart increases so that in a lateral view, the atrium and the ventricle lie adjacent to each other.

  19. Day 8 • STAGE: 37AGE: 7 days

  20. Day 8 Age reason • This is the stage of the Medaka where the pericardial cavity forms. • The tip of the tail lies just past the optic vesicle. • The pharyngeal teeth are visible in the posterior region of the gills between the optic vesicles. • The pericardial cavity surrounding the heart is easily observed. • The slowly moving gut tube has a narrow lumen.

  21. Resources • http://en.wikipedia.org/wiki/Oryzias_latipes • http://www.embl_heidelberg.de/mepd/medakaStages/MedakaDevStages.html • http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/Medaka_stills.html • http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/Medaka_videos.html • https://www.msu.edu/~timkophi/LBS144/medaka.htm • http://www.fishbase.org/Summary/SpeciesSummary.php?id=4669 • http://www.woodrow.org/teachers/bi/1997/medaka/

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