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Molecular Tools

Molecular Tools. Recombinant DNA. Restriction enzymes Vectors Ligase and other enzymes. DNA Cloning. Restriction enzymes Plasmid vector Selectable gene Cloning sites Origin of replication DNA ligase Transformation Competent cells Selection. Dideoxy DNA Sequencing.

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Molecular Tools

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  1. Molecular Tools

  2. Recombinant DNA • Restriction enzymes • Vectors • Ligase and other enzymes

  3. DNA Cloning • Restriction enzymes • Plasmid vector • Selectable gene • Cloning sites • Origin of replication • DNA ligase • Transformation • Competent cells • Selection

  4. Dideoxy DNA Sequencing • Dideoxy nucleotides • Automated DNA sequencers

  5. Polymerase Chain Reaction

  6. Polymerase Chain Reaction • Thermostable DNA polymerase • Oligonucleotide primers • NTPs + buffer • PCR machine

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