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Induction of Apoptosis by SIRT1 and AROS siRNA in Cancer Cell Lines

This study investigates the effects of SIRT1 and AROS siRNA on apoptosis induction in various cancer cell lines, including HCT116, MCF7, WI-38, and MCF10A. The analysis includes mRNA expression levels of SIRT1, AROS, and Lamin A/C, and their correlation with apoptosis rates. The data reveals significant changes in mRNA expression following treatments such as etoposide and TSA, as well as UV-C irradiation. Findings shed light on potential therapeutic targets for enhancing cancer cell apoptosis, contributing to improved cancer treatment strategies.

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Induction of Apoptosis by SIRT1 and AROS siRNA in Cancer Cell Lines

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  1. Supplemental Table 1: Knight et al – Supplementary Table

  2. A B n.s. n.s. ** Fold apoptosis induction - - + + SIRT1 siRNA AROS 2 siRNA Mock - - AROS 1 siRNA + + Fold apoptosis induction Knight et al – Supplementary Figure 1

  3. HCT116 mRNA expression (%) SIRT1 mRNA Lamin AC mRNA AROS mRNA siRNA HCT116 p53-/- MCF7 mRNA expression (%) mRNA expression (%) SIRT1 mRNA SIRT1 mRNA Lamin AC mRNA Lamin AC mRNA AROS mRNA AROS mRNA siRNA siRNA WI-38 MCF10A mRNA expression (%) mRNA expression (%) SIRT1 mRNA SIRT1 mRNA Lamin AC mRNA Lamin AC mRNA AROS mRNA AROS mRNA siRNA siRNA Knight et al – Supplementary Figure 2

  4. A Etoposide and TSA Control Control UV-C Treatment p53 50kDa K382Ac 50kDa * β-actin 37kDa B *** *** *** *** Fold apoptosis vs. Mock Mock Mock SIRT1 Mock Mock SIRT1 AROS AROS siRNA UV-C irradiation Etoposide and TSA Condition Knight et al – Supplementary Figure 3

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