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Characterization of 5’ and 3’ Terminal Sequences of Multiple dsRNA Structures

This study presents the detailed characterization of the 5’ and 3’ terminal sequences of four distinct double-stranded RNA (dsRNA) constructs. Each dsRNA structure is defined by its unique nucleotide composition at both termini, with sequences varying in length and character. Specifically, the 5’ terminal sequences include variants of (C/U)ACGAU, while the 3’ ends include UAA, UGA, and additional nucleotide motifs. Understanding these sequences is crucial for elucidating their functional roles in regulatory RNA processes.

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Characterization of 5’ and 3’ Terminal Sequences of Multiple dsRNA Structures

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  1. Supplementary Fig. 3 (a) 5’-terminal sequences dsRNA1 5’-(C/U)ACGAUUCAACCCACUACUAACUUAAAGUAUC---GUUGAACAUGCAAUCCACUCGUAAG... dsRNA2 5’-(U/C)ACGAUUCAACCCACACAUUGAUCCAUCAAUUCGAAUCACUAUGGCGACCCAAAUGCAAC... dsRNA3 5’-(C/U)ACGAUUCAGCCCCACACUAACCAUGGAUAACCACACCGAUAUUGAGACUGAUACCUACC... dsRNA4 5’-(C/U)ACGAUUCAACCCAACUUUUAGUUCUGCUAUUCGAAUCAACAACAAUGUCCGCACCAAGU... (b) 3’-terminal sequences dsRNA1 UAA..(48 nt).. dsRNA2 UAA..(120nt). dsRNA3 UGA..(59 nt).. dsRNA4 UAA..(166 nt). ACGGGAAACAAA-CAAUACAUAU---UAGCGGAAACGCAAUUCAAUAGAUAUACGUGCUAUUGAUCUUGAGAAUAUUC(G/A)-3’ 4,971 -UGCAAACCAAA-ACAUACAUAU---UAGCGGAAACGCAAUUCAAUAGAUAUACGUGCUAUUGAUCUUGAGAAUAUUC(A/G)-3’ 4,307 GUGUAUAACAAGUCAAUACAUAUUAUCAGCGGAAACGCAAUUCA-UAAGUGCGCGUGACGUUGACUUUGAGAAUAUUC(G/A)-3’ 4,093 -GCUAAACCAAA-ACACACAUAUAAUCAGCGGAAACGCAAUUCAAUAGGUAUACGUGCUAUUGACCAUGAGAAUAUUC(G/A)-3’ 3,468 (c) 5’-(U/C)CACGAUUCAgCC 3’-(A/G)CUUAUAAGAGUU

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