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Comprehensive Laboratory Assistant Training Program

Engage in daily cerebral exercises, watch HCT test demonstrations, and define key terms like Hemoglobin, Leukocyte, and Blood Smear. Learn laboratory skills and state standards for disease prevention strategies. Understand procedures for Hemoglobin tests, Hemolysis, and Blood Smears. Practice interpreting test results and identifying leukocyte types on blood smears. Enhance microscopy skills and specimen collection techniques.

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Comprehensive Laboratory Assistant Training Program

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  1. Students will complete Daily Cerebral Exercise. • Students will watch video of a HCT test being performed. • TO: Define Hemoglobin, Leukocyte, and Blood Smear. • Students will complete Chapter Review for LAS. Turn into class box when finished. 3/19/14 Today’s Agenda: Unit 5: What skills are necessary to be a Laboratory Assistant? Ability to use a microscope. Obtaining specimens. Performing HCT Performing Hemoglobin Test and Blood Smears State Standards: 9.2 Describe strategies for prevention of diseases including health screenings and examinations.

  2. What does a Crit test test for? • In your own words describe the steps for completing and HCT test. • For a skin puncture, why is the first drop of blood not used in testing? • For a culture sensitivity test, what does it mean if the pathogen does not grow up to the antibiotic disc? Daily Cerebral Exercise:

  3. Students will complete Daily Cerebral Exercise. • Students will watch video of a HCT test being performed. • TO: Define Hemoglobin, Leukocyte, and Blood Smear. • Students will complete Chapter Review for LAS. Turn into class box when finished. 3/19/14 Today’s Agenda: Unit 5: What skills are necessary to be a Laboratory Assistant? Ability to use a microscope. Obtaining specimens. Performing HCT Performing Hemoglobin Test and Blood Smears State Standards: 9.2 Describe strategies for prevention of diseases including health screenings and examinations.

  4. Hemoglobin Test (Hgb) • Def – used to determine the oxygen-carrying capacity of bld • Hemoglobin is found on RBC • Composed of two parts: • Heme – iron • Globin – protein • Transports O2 to the body and CO2 to the lungs • Before hemoglobin concentration can be determined, the blood must be hemolyzed.

  5. Hemolysis • Def – destruction of RBCs releases hemoglobin into the solution that surrounds cells. • Normal bld is red & cloudy. • During hemolysis, bld becomes clear.

  6. Two ways to test: both compare hemolysis coloring to get a reading for an individual pt 1. Hemoglobinometer • Not accurate bc relies on human eye. 2. Automated Photometer • Does not depend on human eye, therefore more accurate

  7. Normal Values: • Males = 13 to 18 g of Hgb per 100 mm of bld • Females = 12 to 16 g • Newborns = 16 to 23 g • 1 – 10 y/o = 10 to 14 g Low Hgb = anemia High Hgb = polycythemia

  8. Blood Smear • Def: prepared by placing a small drop of blood on a slide; used for many blood test. • Differential count of WBCs (leukocytes) is one such test. • 100 Leukocytes are counted. • A total of each of the five types of leukocytes is kept. • i.e. 27 lymphocytes = 27% lymphocytes • Increase in WBCs can diagnose infections.

  9. Increase = viral infection, TB Decrease = HIV Increase = bacterial infection Increase = allergic rxn Increase = bacterial infection Increase = Malaria

  10. NAME THAT LEUKOCYTE!

  11. Blood Smear Continued • Used to examine the form, structure, & # of erythrocytes, leukocytes, & platelets. • Abnormal shapes can also be a sign of disease…i.e. Sickle Cell Anemia • Abnormal count & shape can both be seen in types of leukemia.

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