1 / 9

Percival/Thomas: Dual Hypothesis Pathogenesis INTER-RELATIONSHIP

West Virginia University. Biofilm Research Laboratory. Percival/Thomas: Dual Hypothesis Pathogenesis INTER-RELATIONSHIP.

majed
Télécharger la présentation

Percival/Thomas: Dual Hypothesis Pathogenesis INTER-RELATIONSHIP

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. West Virginia University Biofilm Research Laboratory Percival/Thomas: Dual Hypothesis Pathogenesis INTER-RELATIONSHIP CRITICAL COLONIZATION (Anti-Koch) HYPOTHESIS HYPOTHESIS I II BIOFILM RATIO EARLY TISSUE LATE ENDOGENOUS SKIN FLORA EXOGENOUS PATHOGENS Procaryote DEPTH ENVIRONMENT pH/Eh/STRESS 5 – 7 Day Critical Colonization Eucaryote 8 SELECTIVE PRESSURES 1. 2. BI-PHASIC CHRONIC WOUND ENVIRONMENT ORGANISM SELECTION / BF STRUCTURE

  2. West Virginia University Biofilm Research Laboratory Percival/Thomas: Dual Hypothesis Interface of 2 Hypotheses with Wound Development & Treatment Options Reversible Wound Environment pH, Eh Debridement Depth Critical Colonization Hypothesis I (Planktonic) Microbiology Hypothesis II Colonization (Biofilm) Structure (Stage I-II) Breaking the Cycle: Wound Management & Therapeutic Microbiology Debridement Therapeutics Antibiotics (Anti-Tumor) Probiotics

  3. West Virginia University Biofilm Research Laboratory Integrating Biofilm (BF) Stage and Planktonic (P) Transmission“Ping-Pong” Pathogenesis ABIOTIC Eh pH Dressing BF Nutrient Supply & Stress Planktonic Planktonic BF Wound Bed BIOTIC

  4. Biofilm Reactors: Chronic Wounds /Gauze The Covered Wound Bed is a Static Biofilm Reactor Influenced by Eh and pH via Perfusion, Enhancing Up Regulation to Recalcitrant Biofilm Phenotype (PBF) And changing Ratio from Planktonic Phenotype (PP) in Chronic Wounds _ PBF_ PP Planktonic Predominate ≤1 (Susceptible) ≥1 Biofilm Predominate (Recalcitrant)

  5. Tri-Phasic Model West Virginia University Biofilm Research Laboratory

  6. TRI PHASIC (PLUS) WOUND/Perio MODEL pH pH Oxygen tension Procaryote CLSM.SEM RATIO Organisms BF/P Phenotype Zone C Zone B Interface Zone A Target cells Eucaryote Histtoche Live/dead

  7. (CFU’s / ml / cm2) BAD >1 Clinical Outcomes PHENOTYPE Biofilm Planktonic Congo Red+ / Planktonic GOOD <1 Congo Red- / Planktonic (CFU’s / ml / cm2)

  8. West Virginia University Biofilm Research Laboratory COLONIZATION RESISTANCE 1000 µg/ml ANTI-INFECTIVE >5 BAD Clinical Outcomes RATIO MBEC MIC >1 and <5 ANTI-INFECTIVE <1 GOOD MBEC > MIC 0.1 0.01 256 µg/ml

  9. West Virginia University Biofilm Research Laboratory Results

More Related