Methods for Studying Animal Viruses: Challenges and Techniques

Jan 11, 2026

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Studying animal viruses presents greater challenges and expenses compared to bacteriophages. Key methods include cultivation in host cells, living animals, embryonated chicken eggs, and in vitro cell or tissue cultures. Quantitative techniques are essential for virology research, including plaque assays for measuring infectivity, virion counting using electron microscopy, quantal assays to determine infectious doses (ID50, LD50), and hemagglutination assays, notably used in influenza virus studies. These diverse methods are crucial for understanding viral behavior and pathology.

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Methods for Studying Animal Viruses: Challenges and Techniques

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Presentation Transcript

Lab 2
Methods of Study Much more expensive and difficult to study animal viruses than bacteriophages • Cultivation in host cells • Living animal • Embryonated chicken eggs • Cell or tissue culture (= in vitro)
Methods of Study cont: Quantitation • Plaque assay (useful for infective and lytic viruses) • Virion counting with EM • Quantal assay (ID50 or LD50) • Hemagglutination (e.g.influenza virus)