Advanced Reproduction Physiology (Part 4)

1. Advanced Reproduction Physiology(Part 4) Prepared by: A. Riasi http://riasi.iut.ac.ir Isfahan University of Technology College of Agriculture, Department of Animal Science

2. Reference: Pathways to Pregnancy and Parturition (Second revised edition) By: P. L. Senger, 2005 Placentation, the Endocrinology of Pregnancy and Parturition

3. Placenta formation • The phenomenon of intrauterine development ensures that the developing conceptus will receive adequate nutrient and protection during its development.

4. Placenta formation • The placenta is a transient organ of metabolic interchange between the conceptus and dam. • It is also a transient endocrine organ. • The placenta in composed from two parts: • Fetal component derived from the chorion • Maternal component derived from modifications of the uterine endometrium

5. Placenta formation • The functional unit of the fetal placenta is the chorionic villus. • Specific of mare placenta: • It has many specialized microzones of chorionic villi known as microcotyledons • It contains unique transitory structure known as endometrial cups.

6. Placenta formation

7. Placenta formation • Ruminant have cotyledonary placenta. • Placentome consists of: • Fetal cotyledon contributed by the chorion • Maternal cotyledon, originating from caruncular regions of uterus

8. Placenta formation The migrating binucleate giant cells (BGC) in the ruminat placenta

9. Placenta formation • The characteristics and functions of BGC: • They are quite large and two nuclei originated from trophoblast cells. • They are formed continuously throughout gestation. • The BGC migrate from the chorionic epithelium and invade the endometrial epithelium.

10. Placenta formation • The characteristics and functions of BGC (continue): • They are believed to transfer complex molecules from the fetal to maternal placenta. • These cells secrete pregnancy specific protein B (PSPB) and placental lactogen.

11. Placenta functions • The placenta regulate the exchange between fetus and dam. • Placental exchange ways: • Simple diffusion • Facilitated diffusion • Active diffusion

12. Placenta functions • Placental transportation: • Gases • Water • Minerals and vitamins • Glucose and Amino Acids • Proteins • Lipids

13. Placenta functions • Placental transportation (continue): • Large peptide hormones (TSH, ACTH, GH, insulin, glucagon) • Smaller molecular weight hormones (steroids, T3 and T4) and catcholamines • Toxic substances • Bacteria and viruses

14. Placenta functions • In addition to serving as a metabolic exchange organ, the placenta serves as a transitory endocrine organ. • Hormones from the placenta gain accesss to both the fetal and the maternal circulation.

15. Placenta functions • The placenta produces hormones that can affect: • Stimulate ovarian function • Maintain pregnancy • Influence fetal growth • Stimulate mammary function • Assist in parturition

16. Placenta functions

17. Placenta functions

18. Placenta functions

19. Placenta functions • The placenta secretes progesterone and estrogen • Progesterone provides the stimulus for elevated secretion by the endometrial glands • Progesterone inhibits myometrial contraction (progesterone block)

20. Placenta functions

21. Placenta functions • In addition to progesterone, estrogens are also an important product of the placenta. • The placental estrogens are particularly important during the last part of gestation and in most species signals the early preparturient period.

22. Placenta functions • The placenta produce placental lactogen or somatomammotropin. • This hormone have two major effect: • Promoting the growth of fetus • Stimulating the mammary gland of the dam

23. Placenta functions

24. Placenta functions • Relaxin is also a product of placenta. • This hormone is produce in some species: • Humans • Mares • Rabbits • Pigs and monkeys • Cats and dogs

25. Placenta functions • Relaxin cause: • Softening of the connective tissue in the cervix • Promotes elasticity of pelvic ligaments

26. Physiology of parturition • The fetal hypothalamo-pituitary-adrenal axis is obligatory for initiation of parturition. • During the conclusion of gestation, fetal mass approaches the inherent space limitations of the uterus.

27. Physiology of parturition Limited space for fetus Fetus hypothalamus Fetus cortisol Placental estrogen Uterus PGF2α Induction of parturition Progesterone Fetus ACTH

28. Physiology of parturition Plasma cortisol concentration changes during sheep gestation (Yuen et al. 2004)

29. Physiology of parturition • Prepartum increase in sheep circulating cortisol is required for the differentiation and maturation of key fetal organs: • Lung • Liver • Kidney • Brain

30. Physiology of parturition • A positive relationship between the level of activation of the fetal hypothalamus-pituitary-adrenal axis and leptin synthesis and/or secretion in late gestation.

31. Physiology of parturition • The endocrine changes cause two major events to occur: • Removal of the myometrial “progesterone block” enabling myometrial contractions to begin • Increased reproductive tract secretions particularly by the cervix

32. Physiology of parturition • Three stages of parturition are: • Initiation of myometrial contractions • Expulsion of the fetus • Expulsion of the fetal membranes

33. Physiology of parturition • Effect of fetal corticoids: • Converting progesterone to estradiol • Synthesizing PGF2α by placenta • As both estradiol and prostaglandin become elevated, the myometrium becomes increasingly more active • The CL of pregnancy regress

34. Physiology of parturition

35. Physiology of parturition

36. Physiology of parturition Relative hormone profiles in the cow during the periparturient period

37. Physiology of parturition

38. Physiology of parturition

39. Physiology of parturition • Follistatin and activin may be also involved in the mechanism of natural parturition (Xia et al. 2009)

40. Physiology of parturition • In pre-parturition period birth canal is lubricated by: • Mucus secretion in cervix and vagina • Fetal membrane rupture and loss of amniotic and allantoic fluid

41. Physiology of parturition • As the fetus enters the birth canal, it becomes hypoxic • Hypoxia promotes fetal movement that, in turn, promotes further myometrial contraction

42. Physiology of parturition • In most species, expulsion of the fetal membranes quickly follows expulsion of the fetus • It is believed that vasoconstriction of arteries in villi reduces pressure and thus allows the villi to be release from the crypts

43. Physiology of parturition • Prolonged parturition can result in serious complications to both the fetus and dam. • Different causes for dystocia: • Excessive size of fetus • Failure of proper fetal ritation • Multiple births in monotocous species

44. Some research papers associated to this lecture Leibovicha, H., et al. 2001. Effects of recombinant ovine placental lactogen and recombinant ovine growth hormone on growth of lambs and milk production of ewes. Livestock Production Science 68: 79–86. Probo, M., et al. 2011. Peripartal Hormonal Changes in Alpine Goats: a Comparison Between Physiological and Pathological Parturition. Reprod Dom Anim 46: 1004–1010. Leury, B. J., et al. 2003. Effect of insulin and growth hormone on plasma leptin in periparturient dairy cows. Am. J. Physiol. Regul. Integr. Comp. Physiol. 285: 1107–1115. Murawski, M., et al. 2011. Cortisol, progesterone and estradiol secretion in vitro in postpartum placental cotyledons of ewes that gave birth to single or twin lambs. Ann. Anim. Sci., 11: 53–60.