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Protein Digestion in Reproductive Systems by Dr. Nurit Haspel

Explore how proteins are cut and interact in reproductive systems. Learn about Tris, Boric Acid, and EDTA in TBE solution preparation.

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Protein Digestion in Reproductive Systems by Dr. Nurit Haspel

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  1. Dr. NuritHaspelBioinformatist Proteins in Reproductive Systems How do proteins get cut? What proteins does the digested pieces interact with after?

  2. Tris: Keeps the DNA soluble in water • Boric Acid: Is a weak acid • EDTA: binds metal cations, such as Mg2+ Ingredients of TBE and their Functions

  3. 10.8g Tris • 5.5g Boric Acid • 0.93g EDTA PREPARATION OF 100ML OF 10X TBE

  4. C1V1= C2V2 • (10x)(?)= (500ml)(1x) • ?=50ml of 10x TBE stock • 440 ml of MQ to make 500ml PREPARATION OF 500ML 1X TBE FROM 10X STOCK

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