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Understanding Protein Interactions in Reproductive Systems and TBE Preparation

This document provides insights into the role of proteins in reproductive systems, including how proteins are cleaved and the interactions of their digested pieces with other proteins. It also details the preparation of TBE (Tris-Borate-EDTA) buffer, essential for maintaining DNA solubility and binding metal cations. The instructions include ingredient amounts for making 100 ml of 10X TBE, explaining the relationship between concentrations (C1V1=C2V2) and final volume preparation.

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Understanding Protein Interactions in Reproductive Systems and TBE Preparation

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  1. Dr. NuritHaspelBioinformatist Proteins in Reproductive Systems How do proteins get cut? What proteins does the digested pieces interact with after?

  2. Tris: Keeps the DNA soluble in water • Boric Acid: Is a weak acid • EDTA: binds metal cations, such as Mg2+ Ingredients of TBE and their Functions

  3. 10.8g Tris • 5.5g Boric Acid • 0.93g EDTA PREPARATION OF 100ML OF 10X TBE

  4. C1V1= C2V2 • (10x)(?)= (500ml)(1x) • ?=50ml of 10x TBE stock • 440 ml of MQ to make 500ml PREPARATION OF 500ML 1X TBE FROM 10X STOCK

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