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LuminMax-C for High Sensitivity Luminescence Detection

LuminMax-C for High Sensitivity Luminescence Detection. Maxwell Sensors Inc. Who will use ?. Biotechnology research laboratories University biological and biochemical laboratories Hospital research laboratories Government biological and biomedical laboratories

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LuminMax-C for High Sensitivity Luminescence Detection

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  1. LuminMax-C for High Sensitivity Luminescence Detection Maxwell Sensors Inc.

  2. Who will use ? • Biotechnology research laboratories • University biological and biochemical laboratories • Hospital research laboratories • Government biological and biomedical laboratories • Food industry • Environmental testing • Forensic testing

  3. What kind of tests ? • Immunoassay and proteomics • Nucleic acids, DNA, RNA, and genomics • Cell viability tests, luciferase, ATP assay • Clinical diagnostics and tests • E-coli bacterial food tests • Toxin environmental tests • Restaurant sanitary tests

  4. What is Luminescence ? - It generates light by chemical reactions, the light signal can be used for analyte quantification - It does not require external light source to excite the analyte, such as fluorescence or absorbance measurement - Due to photon counting detector can detect a few photons, the detection can be extremely sensitive - Common luminescence are chemiluminescence & bioluminescence

  5. How the light is generated? - There are more than 50 chemical reactions have been used to generate light - The most common reactions use: Substrate + Enzyme excited state molecule*  Light or Substrate + Enzyme + ATP excited state molecule*  Light *excited state molecule: e.g. dioxetane

  6. What are the substrates? There are many different substrates & their derivatives, such as

  7. What are the substrates? There are many different substrates & their derivatives, such as • Luciferin • Acridinium ester • Luminol • Isoluminol • Lumi-PPD,Lumigen-PS-atto, • etc

  8. What are the Enzymes? • Horseradish peroxidase (HRP) • Luciferase • Alkaline phosphatase (AP) • Peroxidase • Dehydrogenase • Galactosidase, etc.

  9. Example 1 Enzyme can be used for quantification

  10. Example 2 Enzyme, ATP, or Mg++ (Ca++) can be used for quantification

  11. How to use for quantification? Enzyme: can be used for indirect labeling proteins or nucleic acids, so it can be used to quantify proteins or nucleic acids Luciferase, ATP, or Ca++ can be used for direct quantification of biological cells

  12. How to label proteins? *: enzyme Light

  13. Enzyme linked immunosorbent assay (ELISA)

  14. Sandwich & Competitive assay

  15. How to label nucleic acids?

  16. How to label nucleic acids?

  17. Glow Luminescence

  18. Flash Luminescence Stable Flash (Promega, Lumigen, etc.) Conventional flash

  19. Luminescence Response Time Lumigen-PS-atto (rapid response)

  20. Sensitivity for Cell Analysis Promega kit

  21. LuminMax-C vs. Beckman-LD400

  22. LuminMax-C System

  23. Luminescent Kits Resources Promega- reagent kits, cell kits Stratagene- reagent kits Bioassay System- luciferase gene assay kits Pierce- enzymes, substrate KPL- enzymes, substrates Lumigen- substrates etc.

  24. Microplate Resources Nunc Costar, Corning, Perkin Elmer, BD Biosciences, Greeiner bio-one Evergreen Sciences Maxwell Sensors Inc. (low cost strip & reusable tray)

  25. Microplate Varieties Types of microplates • Bottom clear: read from bottom (for MSI-LuminMax-C) • Total opaque: need to read from top, signal depends on solution volume Types of surface treatment: • Untreated, Sterile • Treated, Sterile – increase binding • Coating for specific proteins or Nucleic acids

  26. LuminMax-C Advantages • User Friendly: Very easy to use, 5 -10 minutes learning will be able to start to operate. • Cost Effective: The most cost-effective and high performance system on the world market. Offers low cost consumables • High Sensitivity: One of the most sensitive photoncounting luminometer on the market, the system has the ability to detect a few photons or attomoles molecules.

  27. LuminMax-C Advantages (conti.) • Extremely Compact: It is suitable for laboratory uses. • Consistent Testing Results: Signal is sample volume independent. • Minimum Cross-talk: Unique adapter design offers minimal cross-talk and excellent optical efficiency. • Solid Packaged: The system is packaged in metal case; it prevents components damage or mis-alignment during shipping. • Excellent service: Rapid responses (e.g. 24 hours) and immediately actions

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