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Effects of PPARγ Antagonist and NF-κB Inhibitor on Cell Viability in SK-N-SH and MCF10A Cells

Supplementary Figures showing the impact of GW9662 and BAY-11-7082 on cell growth and viability in SK-N-SH and MCF10A cell lines over multiple days. Cell counts were taken to assess cytotoxicity effects.

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Effects of PPARγ Antagonist and NF-κB Inhibitor on Cell Viability in SK-N-SH and MCF10A Cells

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  1. Supplementary Figure 1 x106 SK-N-SH 4 Vehicle 3 GW9662 Number of cells 2 1 0 0 1 2 3 5 7 Day Supplementary Figure 1. Growth of SK-N-SH cells in the presence of absence of GW9962 (15 M). Number of viable cells were measured on the indicated.

  2. Supplementary Figure 2 x105 4.5 4 3.5 3 UT Number of cells 2.5 GW9662 2 BAY-11-7082 Both 1.5 1 0.5 0 0 1 2 3 4 Day Supplementary Figure 2. No cytotoxicity effect of PPAR antagonist (GW9662) and NF- inhibitor (BAY-11-7082) in MCF10A cells. cells were subject to treated (indicated by arrow) by replacing with new media containing either GW9662 (15 M) or BAY-11-7082 (5 M) or both. Cells not treated with either chemicals (UT) were used as comparison. Number of viable cells were measured on 24 hour (Day 2), 48 hour (Day 3) and 72 hour (Day 4).

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