1 / 14

Robotics and Automated Sample Preparation

Robotics and Automated Sample Preparation. January 16, 2009 UNL COBRE retreat Joe Dumais/Kurt Wulser. Facility Goal. Provide a flexible set of sample preparation, identification/tracking and validation tools. Provide the space and support necessary to enable rapid sample generation.

mihaly
Télécharger la présentation

Robotics and Automated Sample Preparation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Robotics and Automated Sample Preparation January 16, 2009 UNL COBRE retreat Joe Dumais/Kurt Wulser

  2. Facility Goal • Provide a flexible set of sample preparation, identification/tracking and validation tools. • Provide the space and support necessary to enable rapid sample generation

  3. Specific Aims • Centralized location for sample production • Provide robotics and instrumentation • Sample tracking/data handling • Provide training for all users

  4. Flow

  5. Development of Lab • Define specific needs of user base • Contact/visit existing facilities to gather information on equipment, personnel and operational issues • Stage purchase of equipment • Develop user training program

  6. Common operations in HTS Labs • Typical sample preparation manipulations • Solvent exchanges • Addition of buffers, target molecules, etc • Concentrate/dilute samples • Microplate centrifuge • Attach sample ID and track samples • Data gathered through well plate readers • Spectrofluorometer/UV-VIS/Luminescence

  7. Facility Personnel Tasks • Maintain systems • Attend vendor training sessions • Track usage • Replenish consumables • Train and assist users • Write programs to execute custom operations • Oversee Facility Budget

  8. Existing High Throughput Laboratories University of Kansas McMaster University, Ontario Vanderbilt

  9. University of Kansas • Established through a COBRE grant targeting cancer research • Focus on screening bioactive compounds against chemical libraries • Staff of 5 • Equipment • 5 plate readers • 3 robots • Bulk reagent dispenser • Plate centrifuge, sealer, handler

  10. McMaster University • Provides biomolecular screening services to researchers across Canada • Staff of 3 people • Sited in 1400 sq. ft lab • Equipment • 2 Liquid Handlers • 5 plate readers • Incubator • Software for database management, statistical analysis and reporting

  11. Vanderbilt University • Provides screening services to academic, government, and commercial agencies. • Staff of 8 people • Equipment • 3 plate readers • 3 bulk liquid handlers • 2 robotic liquid handlers • Robotic arm for transfer of plates to stations • Microplate storage units, centrifuges and plate sealers

  12. Existing UNL Equipment • Genomics Solution Pro Pic robotic workstation • Requires ≈$50K upgrades • Genomics Solutions Investigatator robotic digester • 96 well capacity • Symbiot Robotic Liquid Handler • Used for MALDI plate prep • Waters nanoAQUITY HPLC • 96 well capacity

  13. Proposed New Equipment • Genomics Solution Pro Pic robotic workstation • Requires ≈$50K upgrades • Genomics Solutions Investigatator robotic digester • 96 well capacity • Symbiot Robotic Liquid Handler • Used for MALDI plate prep • Waters nanoAQUITY HPLC • 96 well capacity

  14. Proposed New Equipment • Tecan Freedom Evo 75 robotic workstation • $150-175K • Liquid handling arm • Shaker, incubator, washer, readers (absorbance, fluorescence, luminescence) • Barcode reader software, printer available on request. • Optional robotic gripper available for $24K

More Related