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Endosymbiont Working group: Wolbachia genome analysis

Endosymbiont Working group: Wolbachia genome analysis. Initial research interest: Gene content differences between Wolbachia genomes Functional category comparison between Wolbachia genomes Comparative analysis between Wolbachia genomes and Anaplasma (closely related genus) genomes

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Endosymbiont Working group: Wolbachia genome analysis

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  1. Endosymbiont Working group: Wolbachia genome analysis • Initial research interest: • Gene content differences between Wolbachia genomes • Functional category comparison between Wolbachia genomes • Comparative analysis between Wolbachia genomes and Anaplasma (closely related genus) genomes • Comparative analysis between Wolbachia genomes and host associated [arthropods] metagenome in IMG

  2. Introduction to Wolbachia • Gram-negative bacteria • Produce intracellular inherited infections in many invertebrates • Extremely common with 20-75% of all insects being infected • Infection of numerous non-insect invertebrates including nematodes, mites and spiders • Diverse phenotypes from infection, from endosymbiosis to reproductive parasitism as characterized by their ability to override chromosomal sex determination, induce partenogenesis, selectively kill males, influence sperm competition and generate cytoplasmic incompatibility in early embryos

  3. Wolbachia induced phenotypes Werren et al., 2008, Nature Reviews

  4. Wolbachia genome projects Werren et al., 2008, Nature Reviews

  5. Finished Wolbachia genomes in IMG Wolbachia endosymbiont of Culex quinquefasciatus Pel Wolbachia endosymbiont TRS of Brugia malayi Wolbachia endosymbiont of Drosophila melanogaster Wolbachia sp. wRi

  6. Genome statistics

  7. Endosymbiont Working group: Wolbachia genome analysis • Initial research interest: • Gene content differences between Wolbachia genomes • Functional category comparison between Wolbachia genomes • Comparative analysis between Wolbachia genomes and Anaplasma (closely related genus) genomes • Comparative analysis between Wolbachia genomes and host associated [arthropods] metagenome in IMG

  8. Function category comparison

  9. Wolbachiapan(COG)

  10. Strain-specific COGs Wolbachia (Brugia malayi) • RNase P protein component Wolbachia (Drosophila melanogaster)

  11. Strain-specific COGs Wolbachia (Culex quinquefasciatus_Pel) Wolbachia (wRi)

  12. Wolbachia, endosymbiont of Drosophila melanogaster – no homologs in other Wolbachia genomes

  13. Wolbachia endosymbiont of B. malayi – no homologs in other Wolbachia genomes

  14. Wolbachia endosymbiont of Culex quinquefasciatus – no homologs in other Wolbachia genomes

  15. Wolbachia sp. wRi– no homologs in other Wolbachia genomes

  16. Wolbachia, endosymbiont of Drosophila melanogaster – with homologs in other Wolbachia genomes

  17. Phylogenetic distribution of Wolbachia COGs Anaplasmataceae Rickettsiaceae

  18. Endosymbiont Working group: Wolbachia genome analysis • Initial research interest: • Gene content differences between Wolbachia genomes • Functional category comparison between Wolbachia genomes • Comparative analysis between Wolbachia genomes and Anaplasma (closely related genus) genomes • Comparative analysis between Wolbachia genomes and host associated [arthropods] metagenome in IMG

  19. Introduction to Anaplasma • Gram-negative bacteria • Reside in host red blood cells and lead to the disease anaplasmosis, most commonly in tropical areas of the world • Requires intermediate tick hosts for maturation, and flies may act as mechanical vectors • Species of veterinary interest are found in cattle, sheep and goats

  20. Finished Anaplasma genomes in IMG Anaplasma phagocytophilum HZ Anaplasma centrale str. Israel Anaplasma marginale St. Maries Anaplasma marginale Florida

  21. COGs tree

  22. 16S tree

  23. Whole genome comparison between Anaplasma and Wolbachia Comparison between complete genome of Wolbachia TRS Brugia malayi and Anaplasma phagocytophilum.

  24. Correlation matrix COG

  25. PCA analysis based on COG Anaplasma phagocytophilum HZ Wolbachia TRS Brugia malayi PCA analysis of complete genomes of Wolbachia and Anasplasma species

  26. Comparative analysis between Wolbachia TRS Brugia Malayi and Anaplasma phagocytophilum HZ

  27. COGs in common between Anaplasma spp. and Wolbachia TRS

  28. Correlation based on KO

  29. Pfams of Wolbachia genomes in their family of Anaplasmataceae

  30. Pfams of Wolbachia genomes in their family of Anaplasmataceae helix-turn-helix ankyrin repeat transposase

  31. PCA of Wolbachia genomes Wolbachia sp. wRi Wolbachia endosymbiont of Culex quinquefasciatus Wolbachia endosymbiont of Drosophila melanogaster Wolbachia endosymbiont of TRS of Brugia malayi

  32. Endosymbiont Working group: Wolbachia genome analysis • Initial research interest: • Gene content differences between Wolbachia genomes • Functional category comparison between Wolbachia genomes • Comparative analysis between Wolbachia genomes and Anaplasma (closely related genus) genomes • Comparative analysis between Wolbachia genomes and host associated [arthropods] metagenome in IMG

  33. Comparative analysis between Wolbachia genomes and Host-associated [arthropod] metagenomes

  34. Wolbachia sequences in metagenome dataset • Compared sequenced Wolbachia genomes to Host-associated (arthropod) metagenomes • In the adult Ambrosia beetle metagenome sample from Bern, Switzerland • 36% of the genome from a Culex quinquefasciatus host (mosquito) had hits to the metagenome of ≥90% ID

  35. Horizontal gene transfer • Viruses: • Caudovirales • Eshcherichia phage D108 (Wolbachia TRS) • Kelbsiella phage KP34 (Anaplasma phagocytophilum). • Geminiviridae • Helicobasidium mompa endovirus1 • Secoviridae • Eukaryota • Nematode • Rhaditidae: caenorhabditis elegans bristol 47%

  36. panCOG Gang Mariana, Cristina, Parag, Betsy, Alex, Ananda

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