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Process design for the production of Fab fragment for therapy

Learn about the production process for Fab fragments used in antibody therapy, targeting VEGF to inhibit tumor growth. Discover the benefits of smaller-sized Fab fragments and efficient production techniques like using Hollow-Fiber Bioreactors and Affinity Chromatography.

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Process design for the production of Fab fragment for therapy

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  1. Process design for the production of Fab fragment for therapy Group 6 Shiang-Yuan Hsieh, Yun-Hsuan Liu, Chao-Ming Yen

  2. Antibody therapy

  3. Bevacizumabv.sRanibizumab • Anti-angiogenic drugs: block the formation of new blood vessels to inhibit tumor growth • By targeting and inhibiting the function of a natural protein called vascular endothelial growth factor (VEGF) • The whole antibody versus a antibody fragment

  4. Antibody • Also called immunoglobulins (Ig) that used by immune system. • Five classes of Ig: IgA, IgE, IgG, IgD, IgM.

  5. Fragment antigen binding(Fab fragment) • A region on an antibody which binds to antigens • Composed of one constant and one variable domain of each of the heavy and the light chain

  6. Why make Fab Fragments? • Smaller size that can promote the efficiency of penetration of tissue section. • Reduce nonspecific binding that results from Fc interactions. • Potentially higher sensitivity in antigen. • Reduce the risks from immune response. • Production is more economical.

  7. Production large scale up - Use Hollow-Fiber Bioreactor

  8. Production large scale up - Use Hollow-Fiber Bioreactor

  9. Antibody Purification - Affinity Chromatography Use the original VEGF as the antigens Other antigens (Gammabind G Sepharose) Barbaset al. (2001).

  10. Antibody Purification - Use Device: Gradiflow

  11. Products Recoveries: Affinity Chromatography vsGradiflow

  12. Products Duration

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