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This study explores the significance of Relative Fluorescence Units (RFU) in various biological and chemical assays. By applying a statistical threshold of p < 0.05, we aim to identify meaningful differences in RFU measurements. The research provides insights into the interpretation of fluorescence data, discussing how RFU can be quantitatively assessed in experimental setups. Understanding these metrics enhances data reliability, enabling researchers to draw valid conclusions regarding the behavior of fluorescent compounds or biological samples in response to experimental conditions.
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