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  1. 500 500 400 400 300 300 RFU RFU 200 200 100 100 0 0 0 5 10 15 20 25 30 35 Minutes Upper panel Duplex 7·10-6 Duplex 7·10-5 Duplex 7·10-4 Duplex 7·10-3 Duplex 7·10-2 Duplex 7·10-1

  2. 475 475 450 450 425 425 RFU 400 400 375 375 350 350 0 5 10 15 20 25 30 35 Minutes Zoom 1 Duplex 7·10-6 Duplex 7·10-5

  3. 440 440 420 420 RFU 400 400 380 380 0 5 10 15 20 25 30 35 Minutes Zoom 2 Duplex 7·10-6 Duplex 7·10-5

  4. 450 450 440 440 RFU 430 430 400 400 390 390 420 420 RFU 380 380 370 0 370 5 10 15 20 25 30 35 Minutes 0 5 10 15 20 25 30 35 Minutes Zoom 3 Duplex 7·10-6 Duplex 7·10-5

  5. 432.5 432.5 382.5 382.5 430.0 430.0 427.5 427.5 RFU 380.0 380.0 425.0 425.0 422.5 422.5 377.5 377.5 RFU 420.0 420.0 0 5 10 15 20 25 30 35 Minutes 375.0 375.0 372.5 372.5 370.0 370.0 0 5 10 15 20 25 30 35 Minutes Zoom 4 Duplex 7·10-6 Duplex 7·10-5

  6. 10 10 8 8 6 6 4 4 RFU RFU 2 2 0 0 -2 -2 0 5 10 15 20 25 30 35 Minutes Lower panel Duplex 7·10-6 Duplex 7·10-5