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Mid-labeled Partial Digest

Mid-labeled Partial Digest. Student : 蕭禕廷 Advisor : 傅恆霖 教授. Contents. 1. Introduction 2. Partial Digest 3. Mid-labeled Partial Digest. 1. Introduction. DNA. T C A G G T C A C A. A G T C C A G T G T. Restriction Enzyme ( 限制內切酶 ). EcoRI. G A A T T C. C T T A A G.

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Mid-labeled Partial Digest

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  1. Mid-labeled Partial Digest Student: 蕭禕廷 Advisor: 傅恆霖 教授

  2. Contents • 1. Introduction • 2. Partial Digest • 3. Mid-labeled Partial Digest

  3. 1. Introduction

  4. DNA T C A G G T C A C A A G T C C A G T G T

  5. Restriction Enzyme (限制內切酶) EcoRI G A A T TC C T T A AG

  6. Restriction Sites(切位) EcoRI G A A T TC G A A T TC G A A T TC C T T A AG C T T A AG C T T A AG Restriction Sites

  7. 2. Partial Digest

  8. Partial DigestProblem • Partial digestproblemis to find , by knowing .

  9. Partial DigestProblem Example

  10. Partial DigestProblemAlgorithm • Skiena et al.gave an algorithm

  11. ?

  12. (+

  13. Partial DigestProblemAlgorithm • Each node needs time. • The total cost is . ?

  14. 3. Mid-labeled Partial Digest

  15. Mid-Labeled Partial Digest partial digest

  16. Mid-Labeled Partial Digest • , • contains exactly labels

  17. Mid-Labeled Partial Digest

  18. Mid-Labeled Partial Digest Example

  19. * $ $

  20. $ $ $ $ * $ $

  21. Mid-Labeled Partial Digest Algorithm $

  22. Mid-Labeled Partial Digest Algorithm

  23. Mid-Labeled Partial Digest Algorithm

  24. Mid-Labeled Partial Digest Algorithm

  25. Mid-Labeled Partial Digest Algorithm $ done ?

  26. Mid-Labeled Partial Digest Algorithm . . . . . . . . . . . . . . . . . . . . . . . . . . .

  27. Mid-Labeled Partial Digest Algorithm . . . . . . . . . . . . . . . . . . . . . . . . . . .

  28. Mid-Labeled Partial Digest Algorithm • Each node needs . • The total time is • . Stirling`s approximation : The total time is

  29. Conclusion • For partial digest problem, Skiena et al.gave an algorithm. • For mid-labeled partial digest problem, there is an algorithm for adding labels inside DNA. • for .

  30. References • T. H. Cormen, C. E. Leiserson, R. L. Rivest and C. Stein, Introduction to Algorithms, Second Edition, 2001. • B. Lewin, Genes VII, 2000. • S. S. Skiena, W. D. Smith and P. Lemke, Reconstructing Sets From Interpoint Distances, SOCG, 1990. • D. B. West, Introduction to graph theory, 1996.

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