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Guidelines for Blood Specimen Collection: CLSI Standards Overview (2007)

This document outlines the Clinical and Laboratory Standards Institute (CLSI) procedures for collecting diagnostic blood specimens by venipuncture, as approved in the sixth edition (2007). It details the order of collection for various blood culture tubes, including sodium citrate (blue-top), serum tubes (red, gold, speckled), heparin (green-top), EDTA (lavender, pink, pearl-top), and glycolytic inhibitors (gray-top). Adhering to these standards ensures the accuracy and reliability of laboratory results in clinical settings.

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Guidelines for Blood Specimen Collection: CLSI Standards Overview (2007)

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  1. Order of Collection

  2. Order of DrawCLSI 2007 • Blood Cultures • Sodium Citrate (Blue Top) • Serum tubes (with or without clot activator, with or without gel separator- eg. Red, Gold, or Speckled-Top Tubes) • Heparin (with or without gel- eg. Green Top) • EDTA (Lavender Top, Pink Top or Pearl Top) • Gylcolytic inhibitor (Gray Top) CLSI Procedures for the Collection of Diagnostic Blood Specimens by Venipunture; Approved Standard-Sixth Edition. CLSI documentation H3-A6. Wayne, PA: Clinical and Laboratory Standards Institute; 2007.

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