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5. DNA Sequencing

5. DNA Sequencing. pp 379 - 381. Fredrick Sanger. Developed dideoxy termination sequencing method Nobel Laureate, 1980 Nobel Laureate, 1958; determined insulin sequence. DNA Sequencing. The components of a sequencing reaction are:. dideoxyribose when both oxygen atoms removed.

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5. DNA Sequencing

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  1. 5. DNA Sequencing pp 379 - 381

  2. Fredrick Sanger Developed dideoxy termination sequencing method Nobel Laureate, 1980 Nobel Laureate, 1958; determined insulin sequence

  3. DNA Sequencing The components of a sequencing reaction are:

  4. dideoxyribose when both oxygen atoms removed deoxyribose when oxygen removed 3’ OH functional group necessary for DNA elongation

  5. DNA Sequencing Four reactions are set up; one for each possible nucleotide.

  6. DNA Sequencing components in one test tube

  7. DNA Sequencing DNA Sequencing Video

  8. DNA Sequencing Contents of each tube is separated by gel electrophoresis Which type of gel medium is best suited for this method?

  9. DNA Sequencing

  10. DNA Sequencing

  11. DNA Sequencing

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