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ST-HSC

B-lineage. ST-HSC. B220 +. CLP. T-lineage. Sca-1 low cKit low IL-7R  high Flt3 high TdT +. Lin - Sca-1 high cKit + CD34 + Flt3 - Thy1.1 low. Lin - Sca-1 high cKit + CD34 - Flt3 - Thy1.1 low. CD3  + /CD8  +. MPP. Granulocyte lineage. Sca-1 - cKit + IL-7R  - FcR high

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ST-HSC

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  1. B-lineage ST-HSC B220+ CLP T-lineage Sca-1low cKitlow IL-7Rhigh Flt3high TdT+ Lin- Sca-1high cKit+ CD34+ Flt3- Thy1.1low Lin- Sca-1high cKit+ CD34- Flt3- Thy1.1low CD3+/CD8+ MPP Granulocyte lineage Sca-1- cKit+ IL-7R- FcRhigh CD34+ Gr-1+ GMP Macrophage lineage LT-HSC CMP Lin- Sca-1+ cKithigh Flt3+ Mac1+ Sca-1- cKithigh IL-7R- FcRlow CD34+ Erythroid /Megakaryocyte lineages Sca-1- cKit+ IL-7R- FcRlow CD34- MEP Ter119+ HSCs HPCs Committed Lineage LSK, HSC-enriched Supplemental Figure 1

  2. c-kit 0.2% MPP LSK/MPP A. Vehicle Sca-1 Flt3 0.5% CLP IL-7Ra CLP Sca-1 Flt3 0.6% CMP IL-7Ra CMP Sca-1 FcR CD34 LSK34-/ LSK34+ 0.6% ST-HSC Thy1.1 0.3% LT-HSC Sca-1 Flt3 CD34 Supplemental Figure 2

  3. LSK/MPP c-kit 0.3% MPP B. TCDD Sca-1 Flt3 0.4% CLP CLP IL-7Ra Sca-1 Flt3 0.2% CMP CMP IL-7Ra FcR CD34 Sca-1 LSK34-/ LSK34+ 0.8% ST-HSC Thy1.1 0.2% LT-HSC Sca-1 CD34 Flt3 Supplemental Figure 2

  4. Bone marrow cells Counts (x10-6) C A % Lin- cells 8 LSK MPP 1 B % Bone marrow cells 100 Gr-1 24 CMP Ter-119 20 CLP 2 20 CD3 LSK34- 4 60 B220 2 LSK34+ Days after 5-FU treatment Days after 5-FU treatment Mac-1 30 11 16 16 11 9 0 5 3 9 0 5 3 Supplemental Figure 3

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