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Gene silencing in plants.

Transcription. Transcription. Poly A. Poly A. Poly A. GFP. GFP. GFP. intron. PFG. gene silencing mRNA degradation. Translation. DICER. GFP. RISC. PFG. Poly A. CAP. CAP. CAP. CAP. CAP. Poly A. DICER. GFP. p 35S. p 35S. GFP. intron. PFG. No Fluorescence. Fluorescence.

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Gene silencing in plants.

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  1. Transcription Transcription Poly A Poly A Poly A GFP GFP GFP intron PFG gene silencing mRNA degradation Translation DICER GFP RISC PFG Poly A CAP CAP CAP CAP CAP Poly A DICER GFP p 35S p 35S GFP intron PFG No Fluorescence Fluorescence LB RB LB RB RISC Gene silencing in plants.

  2. UV Nb GFP GFP p 35S p 35S GFP intron p 35S PFG LB LB RB RB LB RB 1.a. Gene silencing of transiently expressedsequences.

  3. UV GFP intron p 35S PFG LB RB GFP 1.b. Triggering gene silencing of established transgene by transient expression.

  4. Nt Nt Nt RZ RZ 16K Agroinfiltration PTGS PDS CP MP pTRV1 RpRd p 35S Sistemic PTGS pTRV2 RB LB LB RB PDS p 35S 1.c. Virus-induced gene silencing (VIGS)

  5. UV GFP GFP intron p 35S PFG LB RB GFP 2. Systemic gene silencing. GFP Agroinfiltration Sistemic PTGS PTGS GFP

  6. HcPro p 35S UV Nb GFP GFP GFP p 35S p 35S p 35S GFP GFP intron intron p 35S p 35S PFG PFG LB RB RB LB LB RB RB LB LB RB LB RB 3.a. Suppressing gene silencing of transiently expressed sequences.

  7. HcPro p 35S UV GFP GFP intron intron p 35S p 35S PFG PFG RB LB LB RB LB RB GFP 3.b.Silencing of trangenes or endogenous genes cannot be established in the presence of silencing suppressor.

  8. PVY infection. Plant silenced for the Magnesium chelatase gene. Plant silenced for the Magnesium chelatase gene. 3.c. Suppression of gene silencing mediated by virus infection.

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