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Steve Meeting January 29, 2003. Took 1 M Betaine data Michelle’s data included in these results. 60 pN / s Bso BI Betaine data. There is a definite difference between 1M betaine and previous data. Note: I shifted the histograms to make the 1M look less “weird”. N=45. N=141.
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Steve Meeting January 29, 2003 • Took 1 M Betaine data • Michelle’s data included in these results
60 pN / s BsoBI Betaine data • There is a definite difference between 1M betaine and previous data. • Note: I shifted the histograms to make the 1M look less “weird” N=45 N=141
Preliminary DFS • Probably would be good to take more data at different rates.
Equilibrium Constant for BsoBI as a function of betaine concentration • I don’t understand the theory enough to interpret the data. • Can be related to # hydrating water molecules released upon binding. • Requires knowing “osmotic pressure” as opposed to molar concentration. • At the very least, it looks like betaine is a great way to increase the EQ constant.