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Effects of Light and Temperature on Photosynthesis in Isolated Chloroplasts

This lab explores how the light reaction of photosynthesis is influenced by the presence or absence of light and the boiling of chloroplasts. We will use DPIP as a substitute for NADP+ to monitor the reduction process through color change from blue to colorless, utilizing a spectrophotometer to measure absorption of blue light. Cuvettes will contain various conditions (normal, boiled chloroplasts, and blank) to investigate their effects on photosynthesis under controlled experimental setups.

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Effects of Light and Temperature on Photosynthesis in Isolated Chloroplasts

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  1. LAB FOUR Photosynthesis

  2. LAB FOUR Photosynthesis 4A Plant Pigment Chromatography 4B Tracking the Light Reaction

  3. LAB FOUR Photosynthesis • How is the light reaction affected by the presence or absence of light? • How is it affected by the boiling of chloroplasts?

  4. LABFOUR Photosynthesis Isolated chloroplasts

  5. LABFOUR Photosynthesis Isolated chloroplasts

  6. LABFOUR Photosynthesis Isolated chloroplasts boiled unboiled

  7. LABFOUR Photosynthesis Isolated chloroplasts boiled unboiled

  8. LABFOUR Photosynthesis Isolated chloroplasts boiled unboiled light dark

  9. LABFOUR Photosynthesis Isolated chloroplasts DPIP

  10. LABFOUR Photosynthesis Isolated chloroplasts DPIP

  11. LABFOUR Photosynthesis Isolated chloroplasts boiled unboiled boiled unboiled

  12. LABFOUR Photosynthesis Isolated chloroplasts boiled unboiled boiled unboiled blank

  13. LABFOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis.

  14. LABFOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless.

  15. LABFOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless. A spectrophotometer measures the absorption of blue light. 0%

  16. LABFOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless. A spectrophotometer measures the absorption of blue light. 10%

  17. LABFOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless. A spectrophotometer measures the absorption of blue light. 40%

  18. LABFOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless. A spectrophotometer measures the absorption of blue light. 80%

  19. LAB FOUR Photosynthesis Cuvettes: • Blank (H2O only) • In the dark • Normal • Boiled chloroplasts • No chloroplasts

  20. LAB FOUR Photosynthesis Cuvettes: Keep all cuvettes on ice!

  21. LAB FOUR Photosynthesis Adjust empty spectrophotometer to 0% Adjust with blank cuvette to 100% Add 3 drops chloroplast to your cuvette and immediately measure transmittance. Measure again at 5, 10, and 15 minutes.

  22. LAB FOUR Photosynthesis • Five teams will handle one cuvette each • Each team will take four measurements • Your turn lasts exactly one minute.

  23. LAB FOUR Photosynthesis Cuvettes: Keep all cuvettes on ice! .

  24. LAB FOUR Photosynthesis Adjust empty spectrophotometer to 0% Adjust with blank cuvette to 100% Add 3 drops chloroplast to your cuvette and immediately measure transmittance. Measure again at 5, 10, and 15 minutes. .

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