1 / 11

- TARGETED ANALYSIS -

sHPP. - TARGETED ANALYSIS -. Adán Alpízar Morúa. Proteomics Unit. Centro Nacional de Biotecnología. Assigned Proteins. Methods (MRM Pilot ). 1. AEAESR. 331.7/462.2. 331.7/591.3. 331.7/488.2. 331.7/401.2. GPLEYPSAK. 530.8/1003.6. 530.8/664.4. 530.8/793.4. 530.8/906.5. 2.

opal
Télécharger la présentation

- TARGETED ANALYSIS -

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. sHPP - TARGETED ANALYSIS - Adán Alpízar Morúa Proteomics Unit. Centro Nacional de Biotecnología

  2. AssignedProteins

  3. Methods (MRM Pilot) 1

  4. AEAESR 331.7/462.2 331.7/591.3 331.7/488.2 331.7/401.2

  5. GPLEYPSAK 530.8/1003.6 530.8/664.4 530.8/793.4 530.8/906.5

  6. 2 QITQVYGFYDECLR (+2) EILVEESNVQR (+2) 896.4/855.4 685.3/861.4 • MCF7 (SDS/PAGE Band 8) P60510 896.4/1059.5 685.3/960.5 896.4/1222.5 685.3/1073.6 QITQVYGFYDECLRK (+3) 640.6/983.5 640.6/1130.5 640.6/1187.5

  7. 3 • PeptideSynthesis Selection Criteria of the Synthetic Peptides • 3-4 peptides per protein • Unique peptides • Peptide Length: 9-21 residues • No missed cleavages • No Mand W • No Qby N-ter • MRM atlas

  8. 3 • PeptideSynthesis

  9. 3 • PeptideSynthesis (MRM)

  10. Conclusions Currently, data depedent analysis yields better results in terms of sensitivity and number of identifications than MRM We have not yet implemented a robust workflow for the routine implementation of MRM methods We have implemented a platform that allows the simultaneous synthesis of a significant (≈ 60) number of peptides

  11. acknowledgments Fernando Roncal Manuel Lombardía Miguel Marcilla SeverineGharbi Carmen González RosanaNavajas Alberto Paradela Salvador Martínez Alberto Medina

More Related