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Quantizzazione di un micro RNA mediante RT-qRCR

Quantizzazione di un micro RNA mediante RT-qRCR. Estrazione RNA. Poliandenilazione mediante Poly-A polimerasi. 5’. AAAAAAAAAAAAAAAAAAAA. 5’. AAAAAAAAAAAAAAAAAAAA. Sintesi cDNA con oligo dT(n). TTTTTTTTTTTTTTTTTTT. 5’. Sintesi filamento complementare al cDNA

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Quantizzazione di un micro RNA mediante RT-qRCR

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  1. Quantizzazione di un micro RNA mediante RT-qRCR Estrazione RNA Poliandenilazione mediante Poly-A polimerasi 5’ AAAAAAAAAAAAAAAAAAAA 5’ AAAAAAAAAAAAAAAAAAAA Sintesi cDNA con oligo dT(n) TTTTTTTTTTTTTTTTTTT 5’ Sintesi filamento complementare al cDNA mediante un oligo specifico TTTTTTTTTTTTTTTTTTT cDNA Amplificazione mediante oligo dT(n) e primer specifico Piastra di caricamento

  2. Curve di amplificazione degli standards

  3. Curva Standard

  4. Curve di amplificazione dei campioni

  5. Report dati Controllo no DNA Standards a conc. nota Campioni Tutti i punti sono in duplicato

  6. FVB = RNA di topi sani (wt) Delta16 no tumor = RNA di topi mutanti ma che non hanno prodotto il tumore Delta16 tumor = RNA di topi mutanti con tumore conclamato

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