1 / 27

Week 6: Cell Morphology

Wright stain RBC morphology Anisocytosis Poikilocytosis WBC morphology. WBC differentials Artifacts. Week 6: Cell Morphology. Wright Stain. Romanowsky stain family Polychromatic Absolute methanol fixative Methylene blue - basophilic components Eosin - acidophilic components.

parker
Télécharger la présentation

Week 6: Cell Morphology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Wright stain RBC morphology Anisocytosis Poikilocytosis WBC morphology WBC differentials Artifacts Week 6: Cell Morphology

  2. Wright Stain • Romanowsky stain family • Polychromatic • Absolute methanol fixative • Methylene blue - basophilic components • Eosin - acidophilic components

  3. Smear Making • Essential in morphology • Wedge method • Thickness and length

  4. Key Features • Size • Nuclear shape • Chromatin features • Granules and other inclusions • Color

  5. Leukocytes • Granulocytes: have specific granules • Eosinophil • Basophil • Neutrophil • Non-granulocytes • Lymphocyte • Monocyte

  6. Granulocytes • Eosinophil • Large orange granules • <2% in circulation • Usually bilobed • Increased in allergy, parasites

  7. Granulocytes • Basophil • Large black-purple granules • <1% in circulation • Increased in allergy, CML

  8. Granulocytes • Segmented Neutrophil • Small purple granules • 54-62% in circulation • Coarse chromatin • Increased in bacterial infection • Band • No nuclear filament • 3-5% in circulation • Increased in bacterial infection

  9. Non-Granulocytes • Lymphocyte • Relatively small • Round or oval nucleus • Smudged chromatin • Basophilic cytoplasm • Increased in viral infection • 25 - 33% (more in children)

  10. Non-Granulocytes • Monocyte • Relatively large • Grey cytoplasm with vacuoles • Irregular nucleus • Linear chromatin • Increased in chronic infection • 3 - 7%

  11. Platelets • Cytoplasmic fragments of large megakaryocyte • 2-3 mm in diameter with granules • Promotes clot formation • 8-20 per oil immersion field • 150,000 - 400,000/mL

  12. Erythrocyte Morphology • Normal: biconcave disc of 7.5 mm diameter • Has central pallor (1/3 of diameter) on smear • No inclusions

  13. Good Area to Evaluate Morphology

  14. Anisocytosis: Variation in Size Microcytes Macrocytes

  15. Poikilocytosis: Abnormal Shape Target Cells Hypochromic

  16. Poikilocytosis Schistocyte Sickle Cell

  17. Poikilocytosis Teardrop Cell Spherocyte

  18. Poikilocytosis Elliptocyte Stomatocyte

  19. RBC Inclusions, etc. Polychromasia Howell-Jolly Bodies

  20. RBC Inclusions Cabot Ring Basophilic Stippling

  21. RBC Inclusions, etc. Malaria Rouleaux

  22. Too Thick Too Alkaline Too Thin Too Acidic

  23. Unacceptable Slides

More Related