html5-img
1 / 82

OSBI Toxicology Lab

OSBI Toxicology Lab. Current Technologies for Blood Testing Robert G. Weston Forensic Toxicologist. We are…. Seven full-time toxicologists One part-time technician Combined 90 years of tox experience. What law enforcement officers do…. Recent DUI caseload. DUI cases submitted:

peggy
Télécharger la présentation

OSBI Toxicology Lab

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. OSBI Toxicology Lab Current Technologies for Blood Testing Robert G. Weston Forensic Toxicologist

  2. We are… • Seven full-time toxicologists • One part-time technician • Combined 90 years of tox experience

  3. What law enforcement officers do…

  4. Recent DUI caseload • DUI cases submitted: • 2011 – 3987 cases • 2010 – 3951 cases • 2009 – 3854 cases • 2008 – 3768 cases • 2007 – 3217 cases

  5. Unlike CSI, we… • must separate the drugs from the blood through sample preparation. • set up the analysis and wait for the data to be collected. • review and interpret the data. • do not know everything about every drug seen in the blood. • determine the identity of substances we haven’t seen before. • do not have a universal test that is effective for all drugs.

  6. Everyone knows how drugs get in…

  7. …but how do we get them out???

  8. Composition of blood • Red blood cells (40-50% of blood volume) • White blood cells (1 % of blood volume) • Platelets • Plasma • Water (>92%) • Sugars • Lipids • Vitamins • Minerals • Hormones • Enzymes • Antibodies • Proteins

  9. Volatiles analysis • Blood placed into a vial • Internal standard is added • Vial is heated • Only volatile components evaporate into the headspace • Alcohols (ethanol, methanol, isopropyl) • Acetone • Toluene • Difluoroethane (from spray dusters)

  10. Volatiles analysis • A portion of the headspace gas is introduced into a gas chromatograph • Comparison is made between the sample and known standards • Quantification of ethanol • Identification of other volatiles

  11. ELISA Enzyme-linked immunosorbent assay

  12. ELISA • Presumptive for classes of compounds • Eight classes currently tested for are • Barbiturates • Benzodiazepines • Carisoprodol/meprobamate • Cocaine/benzoylecgonine • Phencyclidine • Phenethylamines (meth) • Opiates • Tetrahydrocannabinols (marijauna) • Results must be confirmed by a chromatography/mass spectrometry method

  13. At OSBI, all confirmatory testingin toxicology involves… CHROMATOGRAPHY

  14. The separation process Injector Detector MP SP Column

  15. The separation process Injector Detector MP SP Column

  16. The separation process Injector Detector MP SP Column

  17. The separation process Injector Detector MP SP Column

  18. The separation process Injector Detector MP SP Column

  19. The separation process Injector Detector MP SP Column

  20. The separation process Injector Detector MP SP Column

  21. The separation process Injector Detector MP SP Column

  22. The separation process Injector Detector MP SP Column

  23. The separation process Injector Detector MP SP Column

  24. The separation process Injector Detector MP SP Column

  25. The separation process Injector Detector MP SP Column

  26. The separation process Injector Detector MP SP Column

  27. The separation process Injector Detector MP SP Column

  28. The separation process Injector Detector MP SP Column

  29. The separation process Injector Detector MP SP Column

  30. The separation process Injector Detector MP SP Column

  31. The separation process Injector Detector MP SP Column

  32. The separation process Injector Detector MP SP Column

  33. The separation process Injector Detector MP SP Column

  34. The separation process Injector Detector MP SP Column

  35. The separation process Injector Detector MP SP Column

  36. The separation process Injector Detector MP SP Column

  37. The separation process Injector Detector MP SP Column

  38. The separation process Injector Detector MP SP Column

  39. The separation process Injector Detector MP SP Column

  40. The separation process Injector Detector MP SP Column

  41. The separation process Injector Detector MP SP Column

  42. The separation process Injector Detector MP SP Column

  43. The separation process Injector Detector MP SP Column

  44. The separation process Injector Detector MP SP Column

  45. The separation process Injector Detector MP SP Column

  46. The separation process Injector Detector MP SP Column

  47. The separation process Injector Detector MP SP Column

  48. The separation process Injector Detector MP SP Column

  49. The separation process Injector Detector MP SP Column

More Related