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WP9 Analysis for cold tolerance in oat

AveQ - Quedlinburg, 2-4 March 2009. WP9 Analysis for cold tolerance in oat. Institutes involved: CRA-GPG (ex Experimental Institute for Cereal Research), Fiorenzuola d’Arda, Italy Fulvia Rizza, Nadia Faccini, Luigi Cattivelli, Valeria Terzi IPGR, Sadovo, Bulgaria Nadezhda Antonova

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WP9 Analysis for cold tolerance in oat

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  1. AveQ - Quedlinburg, 2-4 March 2009 WP9 Analysis for cold tolerance in oat

  2. Institutes involved: CRA-GPG (ex Experimental Institute for Cereal Research), Fiorenzuola d’Arda, Italy Fulvia Rizza, Nadia Faccini, Luigi Cattivelli, Valeria Terzi IPGR, Sadovo, Bulgaria Nadezhda Antonova Suceava Genebank, Romania Danela Murariu

  3. Frost tolerance field trials: 2008-9 and 2009-10 3 locations Total number of cultivars to be evaluated in the field = 500 Registration of meteo data The field injury will be visually estimated on a 0-9 scale (Rizzaet al.,1994) After beginning the stable vegetable period we have to evaluate survival plants /1m2

  4. 2008-2009 field trials: Environment = Northern Italy The seed set “2008AVEQQualityFRASeeds” was sown in 2.5 msq parcels The field test is ongoing Problem = late sowing

  5. Environment = Romania, Suceava The field test was conducted in 2008 (sowing date 10 April) Problem = high temperatures

  6. Environment = Bulgaria The seed set “2008AVEQQualityDeuSeeds”was sown in autumn 2008 Two repetitions, 50 seeds, 1 m long rows, 20 cm distance between rows Additionally 13 winter accessions sown in parcels (1.5 m x 1 m) The weather was dry, causing some emergence problems. First evaluation on 05.02.2009 The field test is ongoing

  7. 0-9 scale according to Rizza et al. (1994

  8. P8- Lab test for frost resistance. • Low temperatures and light intensity affect the PSII excitation pressure which plants are exposed to and, consequently, their tolerance to stress. • Explore the genetic variability for PSII stability and frost resistance in a set of oat cvs

  9. Chlorophyll fluorescence responds to changes in PSII photochemistry and therefore represents a convenient and rapid tool to evaluate the capacity of the photosynthetic machinery at low temperature. The ratio of variable to maximal fluorescence (Fv/Fm) in dark-adapted state measures the maximum quantum yield for PSII photochemistry and represents a diagnostic probe for measuring low temperature stress-induced injury of photosynthesis = 0.8 in dark-adapted health plants Fv Fm-F0 --- = ---- Fm Fm

  10. Chlorophyll fluorescence evaluation (Fv/Fm) on a set of 100 oat cultivars The standard treatment: 1 week-old plants were hardened at 3-1°C (day/night) 200mE m-2 s-1 for 4 weeks and subjected to freezing treatments (-10°C and –12°C) The Fv/Fm ratios were measured immediately after stress and after recovery.

  11. The best group for frost tolerance selected on the basis of Fv/Fm ratio.

  12. The intermediate group for frost tolerance selected on the basis of Fv/Fm ratio.

  13. The frost susceptible genotypes selected on the basis of Fv/Fm ratio.

  14. Conclusion. • Field tests for cold resistance are ongoing, however we had some problems. • Lab tests based on Fv/Fm ratio evaluation have demonstrated high variability for frost resistance in a set of 100 oat genotypes.

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