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14 C-[AA] (pmol/10 6 cells/min)

A. B. 14 C-[AA] (pmol/10 6 cells/min). PGE2. Vehicle. PGE2. Vehicle. Vehicle. PGE2. PGE2. Vehicle. 1/V (pmol/10 6 cells/min -1 ). 320. 0.064. 0.032. 160. 80. 0.016. C. D. 300. 120. Hydroxyproline ( m g/10 5 cells). ALP (nmol/min/mg prot.). **. 0. **. 25. 50. 100.

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14 C-[AA] (pmol/10 6 cells/min)

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  1. A B 14C-[AA] (pmol/106 cells/min) PGE2 Vehicle PGE2 Vehicle Vehicle PGE2 PGE2 Vehicle 1/V (pmol/106 cells/min-1) 320 0.064 0.032 160 80 0.016 C D 300 120 Hydroxyproline (mg/105 cells) ALP (nmol/min/mg prot.) ** 0 ** 25 50 100 12.5 250 0 0.04 0.08 0.16 0.02 100 ** ** 14C-[AA] (mM) 1/14C-[AA] (mM-1) ** 200 ** 80 ** ** 150 60 * 100 40 * 50 20 0 0 0 7 14 21 0 7 14 28 28 21 Days post confluence H G 14C-[AA] (pmol/106 cells/min) 150 PGE2 (nM) 1 10 100 1000 0 kD ** ** membrane 65 100 * cytosol 65 50 total 65 0 0 1 10 100 1000 PGE2 (nM) Days post confluence E - + PGE2 7 14 21 28 7 14 21 28 Days kD 37 OCN 41 GAPDH F - + PGE2 14 21 28 14 21 28 Days Fig. S3

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