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ELISA Workshop
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Presentation Transcript
1. ELISA Workshop Dr. Mariluci T. Bladon
Director, Biotechnology Program
Middlesex Community College
2. What is ELISA? Enzyme Linked Immunosorbent Assay
Used for detection of infectious diseases, pregnancy, Lyme’s disease and various types of cancer
3. Detection Signals Enzyme-substrate interaction
Fluorescence signal
Radioisotope signal
6. Materials See Handout
96 well plate
7. 96 Well Plate
8. Methods See Handout
12. ELISA Plate Reader
13. Results and Conclusion Role of positive and negative controls
Role of human in error in unexpected results
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